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Detection of HPV by nPCR in oral leukoplakia: case-control study

Grant number: 11/05499-8
Support type:Scholarships in Brazil - Master
Effective date (Start): March 01, 2012
Effective date (End): August 31, 2013
Field of knowledge:Health Sciences - Dentistry - Oral and Maxillofacial Surgery
Principal Investigator:Glauco Issamu Miyahara
Grantee:Lígia Lavezo Ferreira
Home Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The oral leukoplakia is considered as a pre-malignant lesion for the development of the squamous cell carcinoma (SCC), and several risk factors can be related to this carcinogenesis, including the human papilloma virus (HPV). HPV is the main cause of cervical cancer and its role in oral carcinogenesis is still controversial. In the oral mucosa has been made the association of oral leukoplakia with HPV. The aims of this study is to detect the presence of HPV DNA in fresh tissue samples, saliva, plasma and oral exfoliated cells extracted from patients with and without oral leukoplakia, analyzed through the technique of nested PCR (nPCR). For this study will be evaluated 30 patients with oral leukoplakia and 30 patients control patient. Will be performed the extraction of DNA of the samples, and then its amplification through PCR. After confirming the presence and integrity of DNA, will be held nPCR for detection of HPV. The results will be subjected to statistical analysis (chi-square or Fisher's exact test).

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FERREIRA, LIGIA LAVEZO; BIASOLI, EDER RICARDO; BERNABE, DANIEL GALERA; NUNES, CANIS MARONI; MIYAHARA, GLAUCO ISSAMU. Plasma HPV DNA is detectable in oral leukoplakia patients. PATHOLOGY RESEARCH AND PRACTICE, v. 213, n. 7, p. 759-765, 2017. Web of Science Citations: 1.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
FERREIRA, Lígia Lavezo. Detecção do HPV por nPCR em leucoplasias bucais : estudo caso-controle. 2013. Master's Dissertation - Universidade Estadual Paulista (Unesp) Faculdade de Odontologia.

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