Although many studies have been conducted evaluating the neutrophil function in the establishment of visceral leishmaniasis, few are those made with established disease in its different stages.It is possible that some clinical signs such as hemolytic anemia that occurs in the disease in dogs may be related to the oxidative stress resulted from lipid peroxidation.Oxidative stress occurs due to an imbalance between the production of free radicals, which can be derived from activated neutrophils, and the body's antioxidant defenses. These reactive oxygen species injure fundamental cellular structures and cause lipid peroxidation, which, in red blood cells can cause hemolytic anemia. Considering the paucity of researches evaluating the oxidative metabolism and the absence of studies assessing neutrophil apoptosis in canine visceral leishmaniasis and also lack of research correlating neutrophil dysfunction with oxidative stress, this study aims to assess oxidative stress in dogs at different stages of visceral leishmaniasis. To this end, will be comparatively investigated the oxidative metabolism and apoptosis of neutrophils, total antioxidant status and lipid peroxidation of healthy dogs and dogs with visceral leishmaniasis at different stages of the disease as proposed by the Consensus LeishVet. The oxidative metabolism will be assessed by flow cytometry using the probe hidroetidina and by the reduction of nitroblue tetrazolium (NBT). Apoptosis will be measured in capillary flow cytometry using Annexin V-PE system and by morphometry. The antioxidant status will be determined in a spectrophotometer by the method of ABTS and lipid peroxidation will be determined by quantification of malondialdehyde on a ELISA plate reader, both using commercial reagents.
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