The folliculogenesis is a process in which the ovarian follicle develops parallel with the oocyte growing and differentiation, pellucid zone formation and proliferation of granulosa cells (GCs) and raising the teca cells. In common conditions, only one follicle get maturity and only one oocyte is eliminated, while the other follicles goes to atresia. Approximately 20% of couples are infertile and the assisted reproduction techniques, as classical in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI), allows the conception. Innovative technologies as genomics, transcriptomics, proteomics and metabolomics have been used to evaluate the biological systems, including reproductive system. In post-genomic era, the proteomic is a high throughput technology that permit the protein expression evaluation, differential proteomics analysis and genetic function knowledge. Among proteomic strategies, the bidimentional (2D) electrophoresis, liquid chromatography and mass spectrometry are highlighted. As GCs influence the development and maturation of oocytes, this study aims to standardize the proteomic strategy for GCs analysis from patients submitted to IVF. For that, the strategies efficiency will be evaluated, and the strategies arrangement that demonstrate properly will be established for following studies.
News published in Agência FAPESP Newsletter about the scholarship: