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Standardization of proteomics strategy of granulosa cells in patients undergoing in vitro fertilization

Grant number: 11/15210-5
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): March 01, 2012
Effective date (End): February 28, 2014
Field of knowledge:Health Sciences - Medicine - Maternal and Child Health
Principal Investigator:Ismael Dale Cotrim Guerreiro da Silva
Grantee:Mayte Ugeda Silva
Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

The folliculogenesis is a process in which the ovarian follicle develops parallel with the oocyte growing and differentiation, pellucid zone formation and proliferation of granulosa cells (GCs) and raising the teca cells. In common conditions, only one follicle get maturity and only one oocyte is eliminated, while the other follicles goes to atresia. Approximately 20% of couples are infertile and the assisted reproduction techniques, as classical in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI), allows the conception. Innovative technologies as genomics, transcriptomics, proteomics and metabolomics have been used to evaluate the biological systems, including reproductive system. In post-genomic era, the proteomic is a high throughput technology that permit the protein expression evaluation, differential proteomics analysis and genetic function knowledge. Among proteomic strategies, the bidimentional (2D) electrophoresis, liquid chromatography and mass spectrometry are highlighted. As GCs influence the development and maturation of oocytes, this study aims to standardize the proteomic strategy for GCs analysis from patients submitted to IVF. For that, the strategies efficiency will be evaluated, and the strategies arrangement that demonstrate properly will be established for following studies.

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