ROLE OF GM-CSF IN SKELETAL MUSCLE TISSUE REPAIR IN VIVO

• Grant number: 11/15417-9
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2012
• End date: February 28, 2014
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Sang Won Han
• Grantee: Leonardo Martins Silva
• Host Institution: Centro de Terapia Celular e Molecular. Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

The muscle-skeletal lesion are frequentely found in professional sports and recreation being a decisive factor in the abandonment of this practic. The repair of muscle injury is a extensive process, in most cases are imcomplete, due deposition of fibrous mesh. An efficient tissue repair ocorres events as angiogenesis (arteriogenesis and vasculogenesis), fibrogenesis and myogenesis regulated for differents growth factors. GM-CSF is a pleiotropic factor for hematopiesis that stimulates myeloblats and monoblast. Recents results in our laboratory using therapy with humanGM-CSF gene showed proliferation and increased density vessels in small and medium animals with limb isquemic. This work showed a strong therapeutic effect of GM-CSF because it promoted the recovery of muscle mass, force and structure by mobilizing therapeutic cells, augmenting the number of vessels, reduction of fibrosis area and and a improvement in the histopatological profile. These results lead us suppose that GM-CSF can act as a controlling factor and/or regulatory role in the repair process in muscle lesion, acting directly or indirectley in angiogenesis, fibrogenesis ans miogenics mechanismis. In this study we proposed study effects of GM-CSF gene in a regulation of angiogenic, fibrogenesis and myogenic growth factors in muscle lesion murine model.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARTINS, LEONARDO; GALLO, CAMILA CONGENTINO; HONDA, TAMISA SEEKO BANDEIRA; ALVES, PATRICIA TERRA; STILHANO, ROBERTA SESSA; ROSA, DANIELA SANTORO; KOH, TIMOTHY JON; HAN, SANG WON. Skeletal muscle healing by M1-like macrophages produced by transient expression of exogenous GM-CSF. STEM CELL RESEARCH & THERAPY, v. 11, n. 1, NOV 6 2020. (16/04229-0, 11/15417-9, 18/09203-5)