Evaluation of enamel caries-like lesions produced by a S. mutans biofilm

• Grant number: 11/15973-9
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): March 01, 2012
• Effective date (End): February 28, 2014
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Regina Maria Puppin Rontani
• Grantee: Micaela Cardoso
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil

Abstract

Routinely in vitro researches don't draw parallels progression and regression of the initial caries lesion, with clinical protocol parameters for diagnosis of this lesion as ICDAS. This project will be developed in order to identify the effect of demineralization needed to caries lesion in vitro production in bovine enamel, using Streptococcus mutans biofilms with clinical features similar to those found in ICDAS II (2009) criteria, codes 1, 2 and 3. This study aims to test the hypotheses that: i) different demineralization times produce artificial caries lesions comparable to those classified by the ICDAS II codes 1, 2, and 3; will be used the following evaluation parameters to identify the caries production, remineralization and cariogenic challenge: clinical parameters (visual inspection-ICDAS II (2009) and radiographic); morphologicals: topography and depth (MEV, polarized light microscopy, confocal laser scanning) and transverse microhardness (KHN). These parameters will be statistically compared using appropriate statistical test to verify the association between them (p<0,05) (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CARDOSO, Micaela. Production and characterization of enamel caries lesion similar to ICDAS II criteria by Streptococcus mutans biofilm. 2014. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba Piracicaba, SP.