Eucalyptus globulus presents great interest in the forestry sector, especially in relation to the characteristics of its wood to cellulose obtainment. Being considered a sub-tropical species and resistant to low temperatures, has been recommended in the forest sector to replacement the tropical eucalyptus that presents high levels of mortality in colder conditions. Micropropagation has the possibility of propagating selected genotypes without killing the matrix tree, resulting in high multiplication rate, maintaining the genetic selection. E. globulus is a species considered recalcitrant to rooting, especially when it involves adult material, making it difficult to take advantage of the benefits of cloning. Due to the lack of studies involving forms of clonal propagation of this species, this study aims to evaluate micropropagation protocols, establishment phases and in vitro multiplication of gems in order to, not only the clonal propagation of selected matrixes, as also the reversion to juvenility of the material, increasing rooting and survival capacity in the acclimatization phase. To this end, will be evaluated responsive explant to the micropropagation technique, morphology of buds developed, rooting ex vitro and seedlings acclimatization of E. globulus.
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