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Cultivation and phylogenetic study of Mycoplasma bovis isolated of bovine mastitis and study of main in etiological infectious agents mastitis-causing : Staphylococcus aureus and Streptococcus agalactiae

Grant number: 11/16055-3
Support type:Scholarships in Brazil - Master
Effective date (Start): March 01, 2012
Effective date (End): February 28, 2014
Field of knowledge:Agronomical Sciences - Veterinary Medicine
Principal Investigator:Helio Langoni
Grantee:Marcela de Pinho Manzi Zocca
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil


Mastitis is inflammation of the mammary gland, characterized as the most common infectious disease in dairy cattle. This disease causes severe losses, both by the drop in milk production and the quality of milk produced, as the increase in the cost of treatments, and the early disposal of cows who have chronic cases of mastitis. The main agents of contagious mastitis are Staphylococcus aureus, Streptococcus agalactiae and Mycoplasma spp .. Intramammary infection caused by this agent may become a serious problem for dairy herds. It is difficult to treat because the microorganisms are resistant to most antimicrobials used and can become chronic mastitis and high somatic cell count (SCC). In most studies with mastitis, it is reported prevalence of agents such as Streptococcus agalactiae and Staphylococcus aureus, because these agents can be isolated and identified by conventional microbiological methods, however, Mycoplasma bovis requires special conditions. Thus, it is believed that the true prevalence of Mycoplasma bovis is probably underestimated Thus, this study aims to isolate and characterize genotype strains of Mycoplasma bovis from milk samples, thereby determining the actual prevalence of this agent in milk and teats of animals with mastitis, and the study of the etiology of mastitis based on the main infectious agents causing Staphylococcus aureus and Streptococcus agalactiae. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MARCELA P. MANZI; SÂMEA F. JOAQUIM; FELIPE F. GUIMARÃES; ARIANE CRISTINA M.O. BRUDER-NASCIMENTO; JOSÉ CARLOS F. PANTOJA; HELIO LANGONI. Prevalência de Mycoplasma bovis em rebanhos de vacas leiteiras. Pesquisa Veterinária Brasileira, v. 38, n. 4, p. -, Abr. 2018.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
ZOCCA, Marcela de Pinho Manzi. Prevalência de Mycoplasma bovis em rebanhos de vacas leiteiras do interior do estado de São Paulo. 2014. 39 f. Master's Dissertation - Universidade Estadual Paulista "Júlio de Mesquita Filho" Faculdade de Medicina Veterinária e Zootecnia. Botucatu.

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