|Support type:||Scholarships in Brazil - Master|
|Effective date (Start):||March 01, 2012|
|Effective date (End):||February 28, 2014|
|Field of knowledge:||Health Sciences - Medicine - Medical Clinics|
|Principal researcher:||Sergio Catanozi|
|Grantee:||Fernanda Bueno Fusco|
|Home Institution:||Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil|
Changes in lipid as well as carbohydrate metabolism has been shown in the presence of sodium chloride restriction. Hyperlipidemia and the renin-angiotensin system association increases aterogenic process. In this sense, angiotensina II (ANGII) binding to AT1 receptor has been associated to increased lipid infiltration into arterial wall, reactive oxygen species and advanced glycation end products generation, monocyte adhesion in endothelial cells and oxidized-low density lipoprotein (LDL) uptake by macrophage. In this study, experiments are going to be carried out on LDL receptor knockout mice (LDLR KO) on a low salt diet (LS; 0.15% NaCl), as compared with a normal salt diet (NS; 1.27% NaCl). Insertion of a renal artery clip and antihypertensive treatments are going to be carried out in ten-week-old mice. Mice fed LS diet are going to be assigned to groups that receive either losartan (LS+losartan) or hydralazine hydrochloride (LS+hydralazine). The control groups (SHAM) are going to be fed LS or NS diet without both the insertion of renal artery clip and antihypertensive treatment. After 5 months of age, the following parameters will be measured: plasma triglyceride, cholesterol, free fatty acids and aldosterona concentrations, urinary sodium, renal rennin activity, blood pressure, lipid infiltration and macrophage receptor expression in the aortic arch. Thus, it will be possible evaluate early atherosclerotic changes in the vascular wall and relate them to blood pressure and lipemia in hyperlipidemic hypertensive mice fed a low salt diet.