The central nervous system plays complex physiological mechanisms to maintain the balance between food intake and energy expenditure. The hypothalamus integrates multiple central and peripheral influences that control energy homeostasis. Recent studies have shown that mesocorticolimbic regions such as the hippocampus, also participate in the energy balance, overcoming the hypothalamic homeostatic control during consumption of high-fat diet. Chronic consumption of these diets impair the mechanisms involved in energy balance, inducing genesis of obesity. The epigallocatechin-3-gallate (EGCG), the major catechin of green tea, showed anti-obesogenic effects. Here, we aimed to further research this line, already initiated in our laboratory by analyzing the peripheral metabolic effects of EGCG in animal models of high-fat diet induced obesity. Swiss mice will receive high-fat diet for eight weeks and treated with EGCG. Body weight and food intake evolution will be evaluated. In sequence, blood samples will be colected for determination of blood glucose, insulin, leptin, corticosterone and inflammatory mediators levels. The hypothalamus and hippocampus will be dissected and subjected to analysis of western blotting for proteins of the serotonergic system and inflammation. The final data will allow identification of possible mediators of central anti-obesity effects of EGCG.
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