Research of neutralizing antibodies in patients with Hantavirus pulmonary and cardiovascular syndrome and rodents infected with Araraquara Hantavirus

• Grant number: 11/16866-1
• Support type: Scholarships in Brazil - Master
• Effective date (Start): March 01, 2012
• Effective date (End): February 28, 2014
• Field of knowledge: Biological Sciences - Microbiology
• Principal researcher: Luiz Tadeu Moraes Figueiredo
• Grantee: Michelly de Pádua
• Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Abstract:Hantavirus are viruses transmitted by wild rodents. These viruses belong to the family Bunyaviridae, are spherical with a diameter of 73 to 150 nm, with RNA genome of negative polarity, tri-segmented, can cause two serious diseases to humans. Human infection is usually acquired by inhalation of aerosols from excreta of rodents infected with Hantavirus. There are five known Hantavirus in Brazil, causing Cardiovascular and Pulmonary Syndrome (HCPS): Juquitiba, Araraquara, Laguna Negra-like, Castelo dos Sonhos and Anajatuba. Among them Araraquara (ARAV) is found in the region of Ribeirão Preto. In Brazil it is important to the public health the study of viruses associated with rodents, particularly the hantaviruses, because they cause serious disease with high lethality that do not have specific treatment. The recognition of the neutralizing antibodies is important, because in patients with HCPS their presence is positively associated with a better prognosis of the disease. This work aims to standardize techniques of neutralization to the Araraquara hantavirus by plaque reduction and TCID50 in VERO - E6 cells. Then, with the neutralization tests, indeed to test sera from patients with clinical suspicion of hantavirus infection and sera from infected rodents. The results obtained with neutralization tests will be correlated to the outcome of the patients with HCPS in order to obtain a laboratory parameter of disease prognosis.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MACHADO, ALEX MARTINS; DE SOUZA, WILLIAM MARCIEL; DE PADUA, MICHELLY; DA SILVA RODRIGUES MACHADO, ALINE RAFAELA; MORAES FIGUEIREDO, LUIZ TADEU. Development of a One-Step SYBR Green I Real-Time RT-PCR Assay for the Detection and Quantitation of Araraquara and Rio Mamore Hantavirus. Viruses-Basel, v. 5, n. 9, p. 2272-2281, SEP 2013. (11/05067-0, 08/50617-6, 11/16866-1)