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Presence of Enterobacteriaceae and Pseudomonadaceae in neonates oral cavity

Grant number: 11/23244-7
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): February 01, 2012
Effective date (End): January 31, 2013
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Silvana Soléo Ferreira dos Santos
Grantee:Priscila Vitor Alves Ferreira
Host Institution: Instituto Básico de Biociências (IBB). Universidade de Taubaté (UNITAU). Taubaté , SP, Brazil


Members of the Enterobacteriaceae and Pseudomonadaceae families present in the hospital environment can colonize early neonates. This colonization can occur due to aspiration of infected vaginal secretions at delivery, skin contamination, swallowing, or contact with those who take care of the child. It consists of a potential reservoir of microorganisms, oral cavity can be a gateway to these agencies and/or tissues and cause infections of varying severity, because the newborn is more susceptible to the development of disseminated disease, due to barriers anatomical and less effective against infection and immunological immaturity. The aim of this study is to verify the presence of species of the families Enterobacteriaceae and Pseudomonadaceae in the oral cavity of newborns hospitalized in maternity (nursery and rooming-in) and in neonatal ICUs, with and without artificial respirators, in the hands of caregivers and respirators artificial. Also aims to verify the sensitivity of these microorganisms in the biofilm and planktonic form, the antibiotics used in the hospital. Will be included in this study are newborns from 24 to 48 hours. Samples will be collected from the oral cavity of newborns and for those admitted to the neonatal intensive care unit, will also be collected material of artificial respirators and hands of caregivers with sterile swabs soaked in saline solution. The tip of the swab will be cut, placed in buffered saline solution, shaken and the suspension seeded on MacConkey agar and incubated. After growth, will be observed the macro and micromorphological characteristics and obtained pure cultures. The strains are identified by biochemical identification kits. The antimicrobial susceptibility to the microorganisms in their planktonic form shall be verified by agar dilution techniques and selected ESBL strains of Enterobacteriaceae by agar diffusion technique. The senbibilidade biofilms will be evaluated in the microplate and the number of cells / mL and quantified by seeding serial dilutions on agar brain-heart infusion.(AU)

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