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Drug-chitosan complexes added to optical contact lenses based on bacterial cellulose: cytotoxicity, genotoxicity and mutagenicity evaluations

Grant number: 12/00527-6
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): April 01, 2012
Effective date (End): November 30, 2013
Field of knowledge:Biological Sciences - Genetics
Principal Investigator:Ticiana Sidorenko de Oliveira Capote
Grantee:Guilherme Vinícius Do Vale Braido
Home Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

This project is part of a larger research project ever awarded by the Regular Research Grants FAPESP (Process 2011/11810-8). In the last years, biotechnology has advanced in the development of biomaterials for tissue regeneration and for their utilization as medical devices. In this context, reabsorble membranes have shown promising results in tissue regeneration that can be used in the healling of skin wounds, grafts, drug delivery systems and tissue engineering. The Institute of Chemistry at Araraquara - IQ UNESP has developed composites based on bacterial cellulose (BC) for utilization as contact lenses (patent deposited). During the preparation of the lenses are incorporated chitosan nanoparticles in order to increase the transparency of it. Drugs like ciprofloxacin and sodim diclofenac were incorporated into these contact lenses to improve their therapeutic properties. However, in order to ensure the safe use of these composites by the medical device industry, it is necessary to evaluate whether they have cytotoxic, genotoxic and mutagenic activities. This important partnership among researchers from the IQ, FOAr (Araraquara - UNESP) and University of São Paulo (USP) will evaluate cytotoxic, genotoxic and mutagenic potential of these biomaterials. Besides cytotoxicity, it is very important to evaluate the genotoxic and mutagenic potential of these biomaterials, because, if the biomaterials provide such risks, they can induce cellular modifications in the surrounding tissue, with possible progression to tumor formation (carcinogenesis). In vitro tests will be performed to verify if the materials have cytotoxic (XTT and clonogenic survival tests), genotoxic (comet) and mutagenic (micronucleus) potentials. Statistical analysis will be used to assess whether the biomaterials are cytotoxic, genotoxic and/ or mutagenic. This information is essential for the safe release of the biomaterial in future employment in the medical device industry.

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