|Support type:||Scholarships in Brazil - Scientific Initiation|
|Effective date (Start):||April 01, 2012|
|Effective date (End):||December 31, 2013|
|Field of knowledge:||Biological Sciences - Biochemistry - Molecular Biology|
|Principal Investigator:||Cibele Nunes Peroni|
|Grantee:||Eliana Rosa Lima Filha|
|Home Institution:||Instituto de Pesquisas Energéticas e Nucleares (IPEN). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). São Paulo , SP, Brazil|
In our laboratory, a research line in the field of gene therapy for deficiency of growth hormone (GH) is developed. Among the strategies studied, one of them is based on a non-viral technique of direct injection of plasmid DNA (naked DNA), associated with the electrotransfer, using intramuscular administration into dwarf (lit/lit) or immunodeficient dwarf (lit/scid) mice. Thus, the secretion of human growth hormone (hGH) was for the first time maintained for a period of 60 days in the circulation of lit/scid mice, with circulating levels from 1.5 to 3.0 ng hGH/mL followed by a significant weight gain in these treated animals, approximately 30%, compared to the control group (saline injection followed by electroporation). In this project, the goal is to use a homologous system following the electrotransfer of a plasmid containing the gene of the mouse growth hormone (mGH) into dwarf mice (lit/lit). With this model, it is expected to mimic the response that would be obtained in GH-deficient individuals, i.e., administration of a homologous gene into a immunocompetent organism. Please note that this activity is part of a research project recently approved by FAPESP (2010/51116-0).