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Evaluation of hatching rate of mouse tetraploid embryos derived from parthenogenetic activation

Grant number: 11/23890-6
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): April 01, 2012
Effective date (End): March 31, 2013
Field of knowledge:Agronomical Sciences - Veterinary Medicine
Principal Investigator:Marcelo Fábio Gouveia Nogueira
Grantee:Pablo Diego Moço
Home Institution: Faculdade de Ciências e Letras (FCL-ASSIS). Universidade Estadual Paulista (UNESP). Campus de Assis. Assis , SP, Brazil

Abstract

During embryonic development, differentiation processes are consequences of changes occurring into the cells, by transcriptional activities and epigenetic modifications controlled under specific signaling pathways (e.g., the need for biparental genomic complementation of the imprinting phenomenon). It is known that parthenogenesis, tetraploidy and somatic cell nuclear transfer are techniques that alter the epigenetic environment and affect the physiological function of cells and embryonic tissues. This project aims evaluate subsequent changes of the handling of oocyte/embryo (parthenogenesis and tetraploidy, respectivelly) using the Mus musculus as a biological model. For that, hatching rates of tetraploid blastocysts derived from parthenogenetic activation (Group TP) will be compared with rates from parthenogenetic blastocysts (Group PG) and from in vivo fertilization (Group Control). It is expected that hatching rate of the Group TP will not be statistically different from Group PG but both rates will be lower than Group Control.