EFFECT OF PHOSPHODIESTERASE-5 INIBITOR IN ISCHEMIC ACUTE KIDNEY INJURY IN RATS

• Grant number: 11/24028-6
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Effective date (Start): April 01, 2012
• Effective date (End): March 31, 2015
• Field of knowledge: Health Sciences - Nursing - Medical-Surgical Nursing
• Principal Investigator: Maria de Fatima Fernandes Vattimo
• Grantee: Mirian Watanabe
• Host Institution: Escola de Enfermagem (EE). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Ischemic acute kidney injury (AKI) triggers a cascade of events involving inflammatory response and generation of reactive oxygen species that result in endothelial and tubular injury. Moreover, the active mechanism of hypoxia induces adaptation and cytoprotection as the enzyme heme oxygenase-1 (HO-1). The sildenafil citrate confers anti-inflammatory properties and vascular protection. The objective of this study is to evaluate the effect the sildenafil citrate in the LRA in ischemic rats according to the severity of kidney injury. Material and Methods: Wistar rats, adults and males (250 to 300g), divided into the following groups: SHAM (surgery simulation); Sildenafil (sildenafil 0,25 mg/kg, v.o., 1 day); Ischemia 30 min (renal clamping for 30 min); Ischemia 45 min (clamping for 45 min); Ischemia 30 min + sildenafil; Ischemia 45 min + sildenafil. Renal function, oxidative profile, release of nitric oxide (NO), HO-1 and inducible nitric oxide synthase (iNOS) gene expression and histology of renal tissue will be evaluated.

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA FONSECA, CASSIANE DEZOTI; WATANABE, MIRIAN; FERNANDES COUTO, SHEILA MARQUES; CARNEIRO DOS SANTOS, ALEF ARAGAO; BORGES, FERNANDA TEIXEIRA; FERNANDES VATTIMO, MARIA DE FATIMA. The renoprotective effects of Heme Oxygenase-1 during contrast-induced acute kidney injury in pre-clinical diabetic models. Clinics, v. 76, 2021. (11/24028-6, 13/26560-2)

VASCO, CAROLINA FERREIRA; WATANABE, MIRIAN; DA FONSECA, CASSIANE DEZOTI; FERNANDES VATTIMO, MARIA DE FATIMA. Lesão renal aguda induzida pela sepse: efeito de proteção renal dos antioxidantes. Revista Brasileira de Enfermagem, v. 71, n. 4, p. 1921-1927, JUL-AUG 2018. (11/24028-6, 13/26560-2)

CHIMIRRI DE LIMAS MARTINS, ANNA LUIZA; WATANABE, MIRIAN; FERNANDES, SHEILA MARQUES; DA FONSECA, CASSIANE DEZOTI; FERNANDES VATTIMO, MARIA DE FATIMA. Diabetes Mellitus: fator de risco para toxicidade de medicamentos. Revista da Escola de Enfermagem da USP, v. 52, 2018. (11/24028-6, 13/26560-2)

CORDEIRO, PRISCILLA MENDES; FERNANDES, SHEILA MARQUES; DA FONSECA, CASSIANE DEZOTI; WATANABE, MIRIAN; LOPES, SERGIO MARTINS; FERNANDES VATTIMO, MARIA DE FATIMA. Efeito da Justicia acuminatissima, Sara Tudo do Amazonas, na injúria renal aguda isquêmica: estudo experimental. Revista da Escola de Enfermagem da USP, v. 53, 2019. (11/24028-6, 13/26560-2)

FERNANDES, SHEILA MARQUES; CORDEIRO, PRISCILLA MENDES; WATANABE, MIRIAN; DA FONSECA, CASSIANE DEZOTI; FERNANDES VATTIMO, MARIA DE FATIMA. The role of oxidative stress in streptozotocin-induced diabetic nephropathy in rats. ARCHIVES OF ENDOCRINOLOGY METABOLISM, v. 60, n. 5, p. 443-449, OCT 2016. (11/24028-6, 13/26560-2)

FERNANDES VATTIMO, MARIA DE FATIMA; WATANABE, MIRIAN; DA FONSECA, CASSIANE DEZOTI; DE MOURA NEIVA, LUCIANA BARROS; PESSOA, EDSON ANDRADE; BORGES, FERNANDA TEIXEIRA. Polymyxin B Nephrotoxicity: From Organ to Cell Damage. PLoS One, v. 11, n. 8, AUG 17 2016. (11/24028-6, 13/26560-2)

SCHLOTTFELDT, FABIO DOS SANTOS; FERNANDES, SHEILA MARQUES; MARTINS, DANIEL MALISANI; CORDEIRO, PRISCILLA; DA FONSECA, CASSIANE DEZOTI; WATANABE, MIRIAN; FERNANDES VATTIMO, MARIA DE FATIMA. Prevention of amphotericin B nephrotoxicity through use of phytotherapeutic medication. Revista da Escola de Enfermagem da USP, v. 49, n. SI, p. 73-78, FEB 2015. (11/24028-6, 13/26560-2)

MIRIAN WATANABE; GABRIELA FULAN E SILVA; CASSIANE DEZOTI DA FONSECA; MARIA DE FATIMA FERNANDES VATTIMO. NGAL urinária em pacientes sem e com lesão renal aguda em unidade de terapia intensiva. Revista Brasileira de Terapia Intensiva, v. 26, n. 4, p. 347-354, 2014-12-00. (11/24028-6, 10/07656-0)

WATANABE, MIRIAN; DA FONSECA, CASSIANE DEZOTI; FERNANDES VATTIMO, MARIA DE FATIMA. Aspectos instrumentais e éticos da pesquisa experimental com modelos animais. Revista da Escola de Enfermagem da USP, v. 48, n. 1, p. 177-183, FEB 2014. (11/24028-6)

CASSIANE DEZOTI DA FONSECA; MIRIAN WATANABE; SHEILA MARQUES FERNANDES COUTO; ALEF ARAGÃO CARNEIRO DOS SANTOS; FERNANDA TEIXEIRA BORGES; MARIA DE FATIMA FERNANDES VATTIMO. The renoprotective effects of Heme Oxygenase-1 during contrast-induced acute kidney injury in preclinical diabetic models. Clinics, v. 76, 2021-10-18. (13/26560-2, 11/24028-6)

FÁBIO DOS SANTOS SCHLOTTFELDT; SHEILA MARQUES FERNANDES; DANIEL MALISANI MARTINS; PRISCILLA CORDEIRO; CASSIANE DEZOTI DA FONSECA; MIRIAN WATANABE; MARIA DE FATIMA FERNANDES VATTIMO. Prevenção da nefrotoxicidade da anfotericina B por meio do uso de fitomedicamentos. Revista da Escola de Enfermagem da USP, v. 49, p. 74-79, 2015-12-00. (13/26560-2, 11/24028-6)