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In vitro assessment of the cytotoxic and genotoxic effects of absorbable poly-L-lactic acid-derived polymer employed for fabrication of surgery and bucomaxilofacial traumatology materials.

Grant number: 12/01465-4
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Start date: April 01, 2012
End date: December 31, 2012
Field of knowledge:Biological Sciences - Genetics - Mutagenesis
Principal Investigator:Raquel Alves dos Santos
Grantee:Thomás Duzi Martins
Host Institution: Pró-Reitoria Adjunta de Pesquisa e Pós-Graduação. Universidade de Franca (UNIFRAN). Franca , SP, Brazil

Abstract

The searching for new materials with clinical applicability in surgeries and bucomaxilofacial bone reconstructions is relevant if we consider the materials that are used routinely, such as titanium, which limits the process of absorption and osteoinduction. Then, the interest for the poly(L-lactic acid)-derived polymers has been emerged for such applicability due to their biocompatibility, once they exhibit good absorption and low rates of inflammatory response. Even with these advantages, the cytotoxic and genotoxic effects of PLA-derived materials remain unknown. In the present study, the objectives are to evaluate the cytotoxic and genotoxic effects of PLA exposure using an in vitro experimental model with Chinese hamster ovary cell line (CHO-K1). Cytotoxicity will be evaluated by the colorimetric method of XTT and clonogenic assay, while genotoxicity will be evaluated using the alkaline version of comet assay and the micronucleus test in binucleated cells. It will be considered for all analysis the experimental group exposed to titanium discs, as well as the negative and positive controls.

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VEICULO: TITULO (DATA)
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Scientific publications
(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)
UZUN, NELSON; MARTINS, THOMAS DUZZI; TEIXEIRA, GABRIELLA MACHADO; CUNHA, NAYANNE LARISSA; OLIVEIRA, ROGERIO BELLE; NASSAR, EDUARDO JOSE; DOS SANTOS, RAQUEL ALVES. Poly(L-lactic acid) membranes: Absence of genotoxic hazard and potential for drug delivery. Toxicology Letters, v. 232, n. 2, p. 513-518, . (11/21574-0, 12/01465-4, 11/17935-7)