Studies have indicated that it's possible to divert the sexual proportion favoring female bovine embryos produced in vitro, given by semen centrifugation frozen in gradient of such density that sediments X spermatozoon. This diverting has 65% accuracy. It has been shown that many factors could influence in the gender proportion, including: 1 - the insemination timing, 2 - the maturation state of the oocyte in the very moment of in vitro fecundation., 3- in vitro gametes co-incubation timing and 4 - in vitro post-incubation cultivation conditions. The ability of producing as many males as females would be of great importance in the animal production industry as well as the biotechnology programs directed to animal reproduction face many problems, among them, the male production being higher than the female, the purposes of this project are: to try some modifications in all the steps of in vitro production (maturation, fecundation and cultivation) of embryos in order to favor the development of female embryos. The modification made in the fecundation timing and the spermatozoon incubation associated to gender diverting by PercollTM before the fecundation in order to direct the sexual proportion to females.The quality control will be done in every step of the procedures evaluating the motility parameters, vigor and spermatozoon concentration as well as the cleavage rate and in vitro production of the embryos. The sexual proportion will be evaluated by the Polymerase Chain Reaction (PCR), such procedure allows to verify the accuracy of the techniques mentioned above.
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