Concentration of pH, lactate and potassium in bags of dogs red blood cells during storage period

• Grant number: 11/20474-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): April 01, 2012
• Effective date (End): December 31, 2012
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Aline Magalhães Ambrósio
• Grantee: Caroline Yumi Kayano
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

During storage of packed red blood cells (RBC) there may be significant increase in lactate levels for the metabolism of red cell itself, and potassium due to stoppage of the sodium-potassium ATPase, which can be caused by a decrease in pH, allowing the efflux of potassium into the extracellular environment. Thus interfering with the viability of the RBC since its administration can induce the occurrence hyperlactatemia and hyperkalemia, respectively, which can result in deleterious changes in the patient, such as arrhythmia and even death. Despite the importance of knowing the levels of pH, lactate and potassium in the RBC during the storage period so that there aren't hiperlactetemia and iatrogenic hyperkalemia, there are no studies describing specific levels of potassium and lactate in canine blood products. Therefore, this study aimed to determine the pH and the concentration of potassium lactate and bags of RBC canine during the storage period of 21 days in refrigerator. For that, measured concentrations of lactate and potassium in bags of 5 donors dog immediately after collection and after processing and after 7, 14 and 21 days of storage in CPDA-1 bags maintained at 4-6c. The blood test is repeated three times in these same dogs at intervals of 60 days, totaling 15 units of blood analyzed. Therefore, this study aims to evaluate whether there was a significant increase in lactate and potassium in the bag that can cause side effects in patients transfused with this RBC.(AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RODRIGUES, RENATA R.; KAYANO, CAROLINE Y.; DOS SANTOS, VINICIUS P.; MOROZ, LUDMILA R.; FANTONI, DENISE T.; AMBROSIO, ALINE M.. Evaluation of hematologic, biochemical, and blood gas variables in stored canine packed red blood cells, and the impact of storage time on blood recipients. VETERINARY CLINICAL PATHOLOGY, v. 49, n. 2, p. 198-206, JUN 2020. (11/20474-1)

RODRIGUES, RENATA R.; KAYANO, CAROLINE Y.; DOS SANTOS, VINICIUS P.; MOROZ, LUDMILA R.; FANTONI, DENISE T.; AMBROSIO, ALINE M.. Evaluation of hematologic, biochemical, and blood gas variables in stored canine packed red blood cells, and the impact of storage time on blood recipients. VETERINARY CLINICAL PATHOLOGY, JUN 2020. (11/20474-1)