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Serum-free suspension adaptation of human cell lines

Grant number: 12/02109-7
Support type:Scholarships in Brazil - Master
Effective date (Start): May 01, 2012
Effective date (End): February 28, 2014
Field of knowledge:Engineering - Chemical Engineering
Principal Investigator:Kamilla Swiech Antonietto
Grantee:Rafael Tagé Biaggio
Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


The main advantage of using mammalian cells as expression system consists in the fact that these cells are capable of performing post translational modifications as well as protein folding in an authentic way, thus generating a protein with correct therapeutic quality. Nevertheless, a major limitation of using this technology, in comparison to microbial expression system, is the high cost of production due, for example, low expression levels and the need for complex and expensive culture media. Therefore, to have an economically viable production process some points should be carefully analyzed in the choice of the production system. Therefore, the industry, rather than be restricted to the prevailing and consolidated systems, has been searching for new production plataforms. Human cell lines have attracted great interest since they are capable of producing glycosylated proteins in a more similar way to native human proteins. However, due to the recent application, these cells have not been properly characterized in terms of a future commercial application. Based on this, the main objective of this project is to establish serum-free suspension culture for the human cell lines HKB-11, SK-HEP-1 and HepG2. To achieve this, both commercial serum-free medium and the development of proprietary medium will be evaluated. Preliminary studies have been conducted improving the 3 cell lines porposed and it was possible to indentify commercial serum-free formulations. The best resulsts were obtained with HKB-11 cell line.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BIAGGIO, RAFAEL TAGE; ABREU-NETO, MARIO SOARES; COVAS, DIMAS TADEU; SWIECH, KAMILLA. Serum-free suspension culturing of human cells: adaptation, growth, and cryopreservation. Bioprocess and Biosystems Engineering, v. 38, n. 8, p. 1495-1507, AUG 2015. Web of Science Citations: 6.
PICANCO-CASTRO, VIRGINIA; BIAGGIO, RAFAEL TAGE; COVA, DIMAS TADEU; SWIECH, KAMILLA. Production of Recombinant Therapeutic Proteins in Human Cells: Current Achievements and Future Perspectives. PROTEIN AND PEPTIDE LETTERS, v. 20, n. 12, SI, p. 1373-1381, DEC 2013. Web of Science Citations: 13.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
BIAGGIO, Rafael Tagé. Serum-free suspension adaptation of human cell lines. 2014. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto Ribeirão Preto.

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