The epididymis are a structure formed by a curled duct that connects the efferent ducts to the deferens ducts, being divided into segments: head, body and tail. The transit of sperm, that are produced in the testes, along the epididymis promotes morphological and functional changes in the cells, such as changes in the plasma membrane and acrosome, determining, at the end of the process, cells with progressive motility and capable of fertilization, this process is called sperm maturation. Along the way the sperm travels through the epididymis, the fluid is exposed to which undergoes substantial changes in its composition, contributing to the maturation of sperm. The recovery technique of semen directly from the epididymis is a feature that enables the acquisition of genetic material after castration or post mortem, an alternative to the reproduction of animals of high value, besides promote studies on sperm maturation. However, the seminal characteristics of these samples have not yet been precisely determined in dogs, the species of choice as biological models for studies on wild animals and the human epididymis. Against this background, the objective of this research is to promote the study of maturation of canine sperm by analyzing the lipid profile of the plasma membrane of cells by establishing a profile of flagellar activity, mitochondrial and acrosomal during epididymal transit and detecting and quantifying the proteins present epididymal plasma, contributing to more clarifications about the physiological aspects of sperm maturation.
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