The testicular degeneration can lead to subfertility, infertility or sterility of the affected sheep. The main cause of this disease is increased testicular temperature by impaired testicular thermoregulation. Although the treatment consists of removing the causative agent, the prognosis depends on the time of exposure to the offending agent and the degree of injury, thus, agents that stimulate the renewal of the seminiferous epithelium may contribute to the reversal of the situation. Supplementation with vitamin A (Vit. A), vitamin E (Vit. E), selenium (Se) and zinc (Zn), assists in the renewal of the seminiferous epithelium and decreased concentration of reactive oxygen species, so it may be used in the treatment of testicular degeneration, on the other hand the use of laser therapy (LT) of low intensity, which stimulates cellular mitosis, could also serve as an aid in reversal of testicular degeneration. This project aims to recommend a treatment for testicular degeneration in rams using vitamin and mineral supplementation or laser therapy. Will be used 24 sheep divided into 6 treatment groups: 1) control: not degenerate and untreated, 2) does not degenerate and supplemented with vit. A, vit. And, Se and Zn, 3) does not degenerate and subject to LT of low intensity, 4) degenerated and untreated, 5) degenerated and supplemented with vit. A, vit. And, Se and Zn, 6) degenerated and subject to LT of low intensity. During the trial period will be conducted weekly assessments, including: andrological examination, evaluation of testicular temperature and rectal ultrasound assessment of the parenchyma (B-mode) and testicular vascularization (Doppler mode), measurement of testosterone and cortisol. Testicular histopathological are recommended at strategic times. The data will be submitted to analysis of variance employing the statistical program Statistical Analysis System (SAS Institute Inc., 2004), with the added factor repeated measures in time, the chances of interactions with the test time will be determined by the Greenhouse-Geisse , using the repeated command generated by the GLM procedure (SAS PROC GLM). The significance level is 5%.
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