Currently Brazil is the world's largest producer of sugar and ethanol, which is requiring more and more of extensive areas for cultivation of cane sugar. The high production of ethanol industry generates an intense amount of wastes that ends up changing the quality of soil, water and air. This agricultural activity has as pollutants, and pesticides and by-products of burning of cane sugar by-products such as vinasse and filter cake, derived from the distillation process / fermentation and vacuum filtration of sludge retained in the clarifiers. Both the vinasse as filter cake have been used to recovery the soil quality of the crop of cane sugar. However, we dont have a lot of information about its effects in the long run, on the environment, about the care required for the storage of such products, the amount indicated on their application to the culture and if these products cause a deleterious effect on biota exposed at or after its application. Thus, this study aims to examine the biodegradability of the filter cake mixed with soil and crushed sugar cane, through respirometry test of Bartha, and the effects of this toxic waste and their mixtures in test organisms Eisenia andrei ( toxicity test and run) and Allium cepa (chromosome aberrations and micronucleus), and analyze their estrogenic effects, using modified strains of the yeast Saccharomyces cerevisiae. The results of this study will contribute information on the impacts of environmental contaminants by testing the ecotoxicological effects of filter cake from sugar and alcohol industries, these residues in the environment continuously available. It will also be evaluated in case of negative action on the exposed biota, the main mechanisms of action of this residue on the organisms.
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