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In vivo evaluation of ozonated water and Buchenavia tomentosa effect on Candida albicans infection in an experimental prosthetic model

Grant number: 11/08813-5
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): May 01, 2012
Effective date (End): April 30, 2013
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal researcher:CRISTIANE YUMI KOGA ITO
Grantee:Patrícia Carla Lopes
Home Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

Candida spp. are considered important in the etiopathogeny of prosthetic stomatitis in association with other factors. The toxicity of antifungal agents limits the therapeutic options. Then, the search for therapeutic alternatives is constant. The study will evaluate in vivo the activity of the ozonated oil and aqueous extract of Buchenavia tomentosa on the mucosal infection by C. albicans in a experimental prosthetic model (Nett et al., 2010). Ninety rats (Rattus norvegicus Wistar), male, around 350 g, inoculated with standardized suspension of C. albicans ATCC 18804 and divided into 5 groups, according with the treatment (n=18) will be studied: Ozonated oil group; Buchenativa tomentosa group; nystatin (5000 UI/ml); Sodium bicarbonate group (3% in distilled water); and control group (physiologic solution). The animals in each group will be sacrificed after 24, 48 and 72 hours. Microbiological analysis of the biofilm formed on the prosthesis and the histopathological study of the mucosa will be performed. Data of cfu/ml sill be compared with the aid of GraphPad Prism versão 3.02, at 5%. Histopathological findings will be compared among the groups.