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Complete genomes for dengue virus: viral genetic diversity among seropositive first-time blood donors and recipients in two blood centres in Brazil

Grant number: 12/03417-7
Support type:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): June 01, 2012
Effective date (End): May 31, 2017
Field of knowledge:Health Sciences - Medicine - Medical Clinics
Principal researcher:Ester Cerdeira Sabino
Grantee:Antonio Charlys da Costa
Home Institution: Instituto de Medicina Tropical de São Paulo (IMT). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated scholarship(s):14/05211-2 - Viral metagenomics in human plasma of blood donors in two blood centers in Brazil, BE.EP.DD

Abstract

The WHO estimates that 50 million new Dengue Virus (DENV) infections occur worldwide each year resulting in 25,000 to 50,000 deaths. Recently high rates of RNA + donors to DENV observed by our group during epidemics in São Paulo, Honduras and the American Red Cross (American Red Cross - ARC) in Puerto Rico. These studies about viremia in donors were followed up by first well-documented reports of dengue transmission by transfusion in Singapore, Hong Kong and Puerto Rico. Because of high levels of viremia in donations documented during epidemic periods, it is difficult to understand why there are so few descriptions about DENV transmission through blood transfusion. Experience with WNV transmission by blood transfusion, a similar flavovirus carried by arthropods, suggests that Dengue transfusion-transmitted can occur commonly, but low investigated due to lack of active surveillance. As discussed in a recent arbovirus workshop at NIH in 2009 December, questions about the rate of transmission by blood components and clinical consequences of infection by transfusion are keys to guide U.S. and global policies to DENV screening of blood supplies. In this study we will first establish the contents of the serological DENV indicators in donors and recipients in the receptor population with high exposure at the 4 bloodcenters in Brazil of RED-III Brasil to understand the basic indices of infection in these communities and the impact of past transfusions in the seroprevalence. We will set up the infrastructure needed for these kind of studies in Brazil. We will conduct a prospective study also focused to be developed in a dengue outbreak in the geographical area of capitation of one of the bloodcenters and will prepare a collection bound of donor-receivers, which will be tested by a DENV RNA test of high sensitivity to evaluate the transmission rate by transfusion of donations RNA + and the clinical outcome of recipients infection. (AU)

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Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FARIA, N. R.; QUICK, J.; CLARO, I. M.; THEZE, J.; DE JESUS, J. G.; GIOVANETTI, M.; KRAEMER, M. U. G.; HILL, S. C.; BLACK, A.; DA COSTA, A. C.; FRANCO, L. C.; SILVA, S. P.; WU, C. -H.; RAGHWANI, J.; CAUCHEMEZ, S.; DU PLESSIS, L.; VEROTTI, M. P.; DE OLIVEIRA, W. K.; CARMO, E. H.; COELHO, G. E.; SANTELLI, A. C. F. S.; VINHAL, L. C.; HENRIQUES, C. M.; SIMPSON, J. T.; LOOSE, M.; ANDERSEN, K. G.; GRUBAUGH, N. D.; SOMASEKAR, S.; CHIU, C. Y.; MUNOZ-MEDINA, J. E.; GONZALEZ-BONILLA, C. R.; ARIAS, C. F.; LEWIS-XIMENEZ, L. L.; BAYLIS, S. A.; CHIEPPE, A. O.; AGUIAR, S. F.; FERNANDES, C. A.; LEMOS, P. S.; NASCIMENTO, B. L. S.; MONTEIRO, H. A. O.; SIQUEIRA, I. C.; DE QUEIROZ, M. G.; DE SOUZA, T. R.; BEZERRA, J. F.; LEMOS, M. R.; PEREIRA, G. F.; LOUDAL, D.; MOURA, L. C.; DHALIA, R.; FRANCA, R. F.; MAGALHAES, T.; MARQUES, JR., E. T.; JAENISCH, T.; WALLAU, G. L.; DE LIMA, M. C.; NASCIMENTO, V.; DE CERQUEIRA, E. M.; DE LIMA, M. M.; MASCARENHAS, D. L.; MOURA NETO, J. P.; LEVIN, A. S.; TOZETTO-MENDOZA, T. R.; FONSECA, S. N.; MENDES-CORREA, M. C.; MILAGRES, F. P.; SEGURADO, A.; HOLMES, E. C.; RAMBAUT, A.; BEDFORD, T.; NUNES, M. R. T.; SABINO, E. C.; ALCANTARA, L. C. J.; LOMAN, N. J.; PYBUS, O. G. Establishment and cryptic transmission of Zika virus in Brazil and the Americas. Nature, v. 546, n. 7658, p. 406+, JUN 15 2017. Web of Science Citations: 178.
PHAN, TUNG G.; DRENO, BRIGITTE; DA COSTA, ANTONIO CHARLYS; LI, LINLIN; ORLANDI, PATRICIA; DENG, XUTAO; KAPUSINSZKY, BEATRIX; SIQUEIRA, JULIANA; KNOL, ANNE-CHANTAL; HALARY, FRANCK; DANTAL, JACQUES; ALEXANDER, KATHLEEN A.; PESAVENTO, PATRICIA A.; DELWART, ERIC. A new protoparvovirus in human fecal samples and cutaneous T cell lymphomas (mycosis fungoides). VIROLOGY, v. 496, p. 299-305, SEP 2016. Web of Science Citations: 16.
PHAN, TUNG GIA; DA COSTA, ANTONIO CHARLYS; DEL VALLE MENDOZA, JUANA; BUCARDO-RIVERA, FILEMON; NORDGREN, JOHAN; O'RYAN, MIGUEL; DENG, XUTAO; DELWART, ERIC. The fecal virome of South and Central American children with diarrhea includes small circular DNA viral genomes of unknown origin. ARCHIVES OF VIROLOGY, v. 161, n. 4, p. 959-966, APR 2016. Web of Science Citations: 16.
PHAN, TUNG GIA; DA COSTA, ANTONIO CHARLYS; ZHANG, WEN; POTHIER, PIERRE; AMBERT-BALAY, KATIA; DENG, XUTAO; DELWART, ERIC. A new gyrovirus in human feces. VIRUS GENES, v. 51, n. 1, p. 132-135, AUG 2015. Web of Science Citations: 7.
NG, TERRY FEI FAN; ZHANG, WEN; SACHSENROEDER, JANA; KONDOV, NIKOLA O.; DA COSTA, ANTONIO CHARLYS; VEGA, EVERARDO; HOLTZ, LORI R.; WU, GUANG; WANG, DAVID; STINE, COLIN O.; ANTONIO, MARTIN; MULVANEY, USHA S.; MUENCH, MARCUS O.; DENG, XUTAO; AMBERT-BALAY, KATIA; POTHIER, PIERRE; VINJE, JAN; DELWART, ERIC. A diverse group of small circular ssDNA viral genomes in human and non-human primate stools. VIRUS EVOLUTION, v. 1, n. 1 MAR 2015. Web of Science Citations: 25.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
COSTA, Antonio Charlys da. Molecular characterization of full-length dengue virus genome in samples from blood donors and recipients in the states of Pernambuco and Rio de Janeiro. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Medicina Tropical de São Paulo São Paulo.

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