Modulation of anti-inflammatory factors in a model of traumatic injury in rat Achilles tendon

• Grant number: 12/02087-3
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): July 01, 2012
• Effective date (End): June 30, 2015
• Field of knowledge: Health Sciences - Physiotherapy and Occupational Therapy
• Principal researcher: Regiane Albertini de Carvalho
• Grantee: Heliodora Leão Casalechi
• Home Institution: Universidade Nove de Julho (UNINOVE). Campus Vergueiro. São Paulo , SP, Brazil

Abstract

Tendinitis is a common disorder of the musculoskeletal system and has various clinical manifestations. Although most tendons have the ability to spontaneously repair after injury, scar tissue formed may impair the normal tendon functioning. Studies have been conducted using phototherapy in different areas of health, using both lasers and LEDs of low intensity. However, there is a small number of studies concerning on the use LEDs for the treatment of musculo-skeletal diseases. The main purpose of this study is to investigate the effect of LED phototherapy in the process of tendon repair after experimental induction of rat Achilles tendonitis. The experimental model of rat Tendinitis consists of a trauma induced to the Achilles tendon of the animals, using an special apparatus. Approximately 112 male Wistar rats will be used(240 ± 20g), The animals will be divided into 14 groups and two different experimental periods (7 and 14 days).. The LED phototherapy will be applied transcutaneously on the injured area. The control animals will be submitted to the simulation of the application with the power turned off. The euthanasia will occur at 7 and 14 days after the induction of tendinitis. Microscopical analysis will be performed in order to evaluate inflammatory cells migration andcollagencontents and organization. Besides, vascular permeability and the inflammatory mediators gene expression will be verified quantified by RT-PCR.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CASALECHI, HELIODORA LEAO; PINTO LEAL-JUNIOR, ERNESTO CESAR; XAVIER, MURILO; SILVA, JR., JOSE ANTONIO; DE TARSO, PAULO; DE CARVALHO, CAMILLO; AIMBIRE, FLAVIO; ALBERTINI, REGIANE. Low-level laser therapy in experimental model of collagenase-induced tendinitis in rats: effects in acute and chronic inflammatory phases. Lasers in Medical Science, v. 28, n. 3, p. 989-995, MAY 2013. (12/02087-3, 11/05890-9)