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Cytotoxicity ánd solubility of experimental retrofilling materials associated with MTA

Grant number: 12/03406-5
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): June 01, 2012
Effective date (End): December 31, 2012
Field of knowledge:Health Sciences - Dentistry - Endodontics
Principal Investigator:Carlos Henrique Ribeiro Camargo
Grantee:Vanessa Megumi Shiomi
Home Institution: Faculdade de Odontologia (FOSJC). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil


The aim of this study is to evaluate the cytotoxicity and solubility of the mineral trioxide aggregate (MTA - Angelus, Londrina - PR, Brazil) and associations with AH Plus (Dentsply, Brazil); castor-oil bean based experimental sealer (Polifil - Araraquara Polímeros Quimicos Ltda., Araraquara - SP, Brazil) and Copaifera sp. oil-resin based experimental sealer (Cop Endo, Brazil). For the cytotoxicity test, sealers will be prepared and kept in contact with the culture medium according to the setting time (0 hour, 24 hours, 7 days and 30 days). Chinese hamster fibroblasts (V79) will be exposed to different dilutions of the sealers extracts for 24 hours and the cell viability will be assessed by MTT test. For the solubility test, 12 specimens of each retrofilling sealer will be prepared using teflon molds. The specimens will be stored in the oven at 37°C with relative humidity of 100% for 7 days for the completed setting. The specimens will be weighted and kept in capped bottles with distilled water in the oven for 24 hours. After that, they will be dried and weighted again. The same procedure will be repeated for periods of 48 hours, 1 week and 1 month. The results will be analyzed by the statistical tests: ANOVA and Tukey, with 5% significance (p<0,05).