Study of involvement of cannabinoid and serotonergic neurotransmission and theirs CB1 and 5-HT1A receptors in sweet-substance-induced antinociception

• Grant number: 12/07225-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date until: June 01, 2012
• End date until: May 31, 2013
• Field of knowledge: Biological Sciences - Pharmacology - Neuropsychopharmacology
• Principal Investigator: Norberto Cysne Coimbra
• Grantee: Felipe Alves de Oliveira
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

This work aims to investigate the action of CB1 cannabinoid receptors and the serotonin 5-HT1A antinociception caused by the ingestion of sucrose, by testing tail-flick latency (TLRC) in animal models. As part of the study, cannabinoid neurotransmission will be investigated from the intraperitoneal administration of increasing doses of agonists and antagonists of the CB1 cannabinoid receptor, respectively, anandamide and AM251, then with oral administration of 250 g / L sucrose 10 minutes after . Similarly, the serotonergic neurotransmission will be investigated by intraperitoneal administration of increasing doses of drug methysergide, the non-selective antagonist of the serotonin receptor, and also drug WAY100635, a selective antagonist at the 5-HT 1A serotoninergic receptor, with the oral treatment of a sucrose solution 250g / L, after 10 minutes. And finally, the most effective doses of cannabidiol, methysergide, and WAY100635 will be tested together in search of an association between the receptors involved. The implications of such work may shed more robust neuropharmacological explanations about the antinociception of sweet substance-induced, resulting for example in anti-pain and anti-stress, especially in newborns.(AU)