Molecular typing of Staphylococcus aureus by MLST and SPA typing and identification of Staphylococcus coagulase-negative by MALDI-TOF isolated from bovine milk

• Grant number: 12/05967-4
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: September 01, 2012
• End date: November 30, 2012
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Maria de Lourdes Ribeiro de Souza da Cunha
• Grantee: Patrícia Yoshida Faccioli Martins
• Supervisor: Jaap A. Wagenaar
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Institution abroad: Utrecht University (UU), Netherlands
• Associated to the scholarship: 10/17222-8 - Molecular epidemiology of oxacillin-resistant Staphylococcus aureus and coagulase negative staphylococci from bulk tank milk of dairy herds., BP.PD

Abstract

Milk is an important food for man, and its quality must be appropriate, without transmit biological hazards. For this, the herd health must be ensured, and studies about the quality of the produced milk are relevant. Staphylococcus genus is the most frequently found in milk. The S. aureus pathogenicity is already known and the staphylococcus coagulase-negative pathogenicity has been studied. Several studies in animals have been done to define their importance in public health as transmitter of these bacteria, mainly methicillin-resistant, which are pathogens responsible for different kinds of infections in humans with difficult cure and they can cause death. Considering the cited points, the aims of this study are perform molecular typing of Staphylococcus aureus methicillin-resistant for identification of sequence types and their respective clonal complex by MLST and spa typing, determine the cromossomic cassette types not characterized, and identify the staphylococcus coagulase-negative by MALDI-TOF technique, and to compare the results with the conventional biochemical identification. The reason for this project is to improve the epidemiology study of Staphylococcus methicillin-resistant from animals, with the training in modern techniques of molecular typing and in bacterial identification, in renowned research institute in the Netherlands, and consequently the group works can have an improvement in quality and the possibility of publication in international periodicals of excellence. (AU)