Action of macrophages treated with crotoxin on the secretory activity of neutrophils in co-culture model

• Grant number: 12/08880-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: July 01, 2012
• End date: December 31, 2012
• Field of knowledge: Biological Sciences - Pharmacology - General Pharmacology
• Principal Investigator: Sandra Coccuzzo Sampaio Vessoni
• Grantee: Renata Begliomini Bruno de Oliveira
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

Crotoxin (CTX), the major toxin of Crotalus durissus terrificus venom induces anti-inflammatory and immunomodulatory effects. Despite the inhibitory actions on neutrophil migration and the phagocytic activity of these cells, this toxin does not alter the microbicidal activity of neutrophils activated by PMA. On the other hand, CTX enhances the generation of reactive oxygen intermediates and nitrogen by macrophages, cells central to innate defense mechanisms. Recent studies have shown the importance of testing in co-culture, showing the interaction between macrophages and neutrophils and the importance of this interaction in the activity of functional and secretory of these cells in the control of the inflammatory response. Considering these stimulatory actions of CTX on macrophages and the importance of cooperation between macrophages and neutrophils in adaptive antimicrobial defense is relevant to investigate the actions of macrophages treated with CTX on H2O2 and HOCl release and antimicrobial activity of neutrophils in a co-culture model. This study contributes to the elucidation of the mechanisms involved in the modulatory actions of CTX on the inflammatory response.(AU)