The knowledge about organogenesis of fish larvae is a useful tool to establish their functional capabilities and systemic physiological needs in order to ensure welfare and optimal growth under aquaculture conditions. Feed in hatchery affects digestive functions inducing faster or slower development. This project aims to evaluate growth, morphophysiological development of the gastrointestinal tract and the molecular ontogeny of the digestive capacity in pacu Piaractus mesopotamicus larvae, through the analysis of production performance, histology, histochemistry, enzimology and molecular biology. Larvae will receive live food and formulated diet with different weaning protocols. Activities of gastric (pepsin), pancreatic (trypsin, chymotripsin, amylase and lipase) and intestinal enzymes (alkaline phosphatase, aminopeptidase and maltase) will be analyzed. Further, analysis of differential expression (trypsin, amylase, lipase, pepsin, cholecystokinin and ghrelin) during larval development will be performed. Therefore, this research will contribute to the better understanding of the development of the gastrointestinal tract in pacu larvae and its digestive capacity, using histology, histochemistry, enzimology and molecular biology approaches. This biology molecular approach never used for neotropical fish larvae.
News published in Agência FAPESP Newsletter about the scholarship: