Digestive capacity during ontogeny of pacu Piaractus mesopotamicus larvae

• Grant number: 11/18607-3
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: August 01, 2012
• End date: February 28, 2014
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
• Principal Investigator: Maria Célia Portella
• Grantee: Thiago Mendes de Freitas
• Host Institution: Centro de Aquicultura (CAUNESP). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The knowledge about organogenesis of fish larvae is a useful tool to establish their functional capabilities and systemic physiological needs in order to ensure welfare and optimal growth under aquaculture conditions. Feed in hatchery affects digestive functions inducing faster or slower development. This project aims to evaluate growth, morphophysiological development of the gastrointestinal tract and the molecular ontogeny of the digestive capacity in pacu Piaractus mesopotamicus larvae, through the analysis of production performance, histology, histochemistry, enzimology and molecular biology. Larvae will receive live food and formulated diet with different weaning protocols. Activities of gastric (pepsin), pancreatic (trypsin, chymotripsin, amylase and lipase) and intestinal enzymes (alkaline phosphatase, aminopeptidase and maltase) will be analyzed. Further, analysis of differential expression (trypsin, amylase, lipase, pepsin, cholecystokinin and ghrelin) during larval development will be performed. Therefore, this research will contribute to the better understanding of the development of the gastrointestinal tract in pacu larvae and its digestive capacity, using histology, histochemistry, enzimology and molecular biology approaches. This biology molecular approach never used for neotropical fish larvae.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE FREITAS, THIAGO MENDES; LOPES, IVA GUIDINI; LOPES, TAIS DA SILVA; BUZOLLO, HELLEN; PORTELLA, MARIA CELIA. GROWTH PERFORMANCE AND INCIDENCE OF SKELETAL ANOMALIES IN PACU LARVAE UNDER DIFFERENT WEANING PROTOCOLS. BOLETIM DO INSTITUTO DE PESCA, v. 45, n. 1, 2019. (11/18607-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

FREITAS, Thiago Mendes de. Capacidade digestiva durante a ontogenia de larvas de pacu Piaractus mesopotamicus. 2015. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.