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Identification of cis-acting elements and transcription factors controlling the expression of sugarcane (Saccharum spp.) 1-aminocyclopropane-1-carboxylic acid synthase

Grant number: 12/06906-9
Support type:Scholarships in Brazil - Master
Effective date (Start): August 01, 2012
Effective date (End): February 28, 2014
Field of knowledge:Biological Sciences - Genetics - Plant Genetics
Principal Investigator:Marcelo Menossi Teixeira
Grantee:Valter Miotto Alessio
Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Ethylene is a plant hormone involved in the developmental regulation and in biotic and abiotic stress signaling. The conversion catalyzed by ACS enzyme (1-aminocyclopropane-1-carboxylic acid synthase) has been reported as a limiting step of ethylene biosynthesis. In sugarcane it is usual the application of ethylene precursors as a way to accelerate the maturation process, although the mechanisms of action in this process remain unknown. Therefore, a way to improve our knowledge in this question is the identification of the elements that control the expression of key genes as ACS. The aim of this study is to identify the cis-acting regulatory elements and transcription factors controlling the expression of two ACS coding genes of sugarcane. The identification of these elements will bring new insights in the sugarcane ethylene biosynthetic regulatory network and new perspectives for further functional studies of these regulatory genes.