Investigation of the role of cyclooxygenase-2 in periapical bone resorption induced by bacterial lipopolysaccharide

• Grant number: 12/08421-2
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: August 01, 2012
• End date: July 20, 2014
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Francisco Wanderley Garcia de Paula e Silva
• Grantee: Fernanda Regina Ribeiro Santos
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 10/17611-4 - Mechanisms involved in the regulation of 5-lipoxygenase pathway in experimentally-induced apical periodontitis, AP.JP

Abstract

During the inflammatory response, several mediators are released locally with order to stimulate the humoral and cellular immune response. Through the action of the enzyme cyclooxygenase-2 structural changes occur in the chain of arachidonic acid leading to the synthesis of prostaglandins responsible for regulating the potential bone metabolism during inflammatory events. Thus, the objective of this study will evaluate the role of the enzyme cyclooxygenase-2 in bone resorption and periapicalexpression of genes involved in bone metabolism, after inoculation of bacterial lipopolysaccharide(LPS) in the root canals of molars of mice. One hundred and twenty C57BL/6 mice will be used. Root canals will be inoculated with a solution containing bacterial lipopolysaccharide from E. coli (1.0 mg / ml). After experimental periods of 7, 14, 21 and 28 days, the animals will undergo euthanasia and the blocks containing teeth and bone will be removed for histology and RNA extraction. Histological sections will be made, stained with hematoxylin and eosin, and for immunohistochemistry for COX-2, RANK, RANKL and OPG and analyzed by conventional light microscopy and fluorescence. Then gene expression will be assessed by real time reverse transcription polymerase chain reaction (qRT-PCR) using the TaqMan ® system and by means of a PCR Array (Osteogenesis RT PCR² Profiler Array. Data obtained will be statistically analyzed according to the data distribution.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RIBEIRO-SANTOS, FERNANDA REGINA; ARNEZ, MAYA FERNANDA MANFRIN; DE CARVALHO, MARCIO SANTOS; DA SILVA, RAQUEL ASSED BEZERRA; POLITI, MARILIA PACIFICO LUCISANO; DE QUEIROZ, ALEXANDRA MUSSOLINO; NELSON-FILHO, PAULO; DA SILVA, LEA ASSED BEZERRA; FACCIOLI, LUCIA HELENA; PAULA-SILVA, FRANCISCO WANDERLEY GARCIA. Effect of non-steroidal anti-inflammatory drugs on pulpal and periapical inflammation induced by lipopolysaccharide. CLINICAL ORAL INVESTIGATIONS, v. 25, n. 11, p. 6201-6209, NOV 2021. (12/08421-2, 10/17611-4)

RIBEIRO-SANTOS, FERNANDA REGINA; ARNEZ, MAYA FERNANDA MANFRIN; DE CARVALHO, MARCIO SANTOS; DA SILVA, RAQUEL ASSED BEZERRA; POLITI, MARILIA PACIFICO LUCISANO; DE QUEIROZ, ALEXANDRA MUSSOLINO; NELSON-FILHO, PAULO; DA SILVA, LEA ASSED BEZERRA; FACCIOLI, LUCIA HELENA; PAULA-SILVA, FRANCISCO WANDERLEY GARCIA. Effect of non-steroidal anti-inflammatory drugs on pulpal and periapical inflammation induced by lipopolysaccharide. CLINICAL ORAL INVESTIGATIONS, APR 2021. (12/08421-2, 10/17611-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SANTOS, Fernanda Regina Ribeiro. Role of cyclooxygenase in inflammation and periapical bone resorption induced by bacterial lipopolyssacharide. 2015. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Odontologia de Ribeirão Preto (PCARP/BC) Ribeirão Preto.