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Development of immunosensors array to detect pathogenic microorganism in foods using microfluidic system.

Grant number: 12/09752-2
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): August 01, 2012
Effective date (End): July 31, 2014
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
Principal researcher:Ronaldo Censi Faria
Grantee:André Santiago Afonso
Home Institution: Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil


The purpose of this project is developing a sandwich imunossensores array for multiplexed detection of pathogenic bacteria in foods using a microfluidic system. The pathogenic bacteria are the major causative agents of foodborne disease (FBD). The sensing device will be developed using primary antibodies immobilized on a film layer-by-layer (LBL) formed by gold nanoparticles (AuNPs) and políons on a graphite electrode array coupled to a microfluidic system. Massively modified magnetic particles with HRP, as electrochemical label, and the secondary antibodies are used to capture and pre-concentration of bacteria. As transduction system will evaluate the use of multiplex techniques voltammetry or chronoamperometry. For characterization of the films and the process of antibody immobilization techniques will be used for specular reflectance spectroscopy in the infrared, atomic force microscopy (AFM) and electrochemical impedance spectroscopy (EIS). The developed system will be evaluated using real samples spiked with DTA. The device will detect DTA at low detection limits, with high selectivity and low cost.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
AFONSO, ANDRE S.; ULIANA, CAROLINA V.; MARTUCCI, DIEGO H.; FARIA, RONALDO C. Simple and rapid fabrication of disposable carbon-based electrochemical cells using an electronic craft cutter for sensor and biosensor applications. Talanta, v. 146, p. 381-387, JAN 1 2016. Web of Science Citations: 24.

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Filed patent(s) as a result of this research project

DISPOSITIVO MICROFLUÍDICO E MÉTODO DE FABRICAÇÃO DE DISPOSITIVO MICROFLUÍDICO BR1020160109841 - Universidade Federal de São Carlos (UFSCAR) . Ronaldo Censi Faria; Carolina Venturini Uliana; André Santiago Afonso - May 2016, 13

DISPOSITIVO MICROFLUÍDICO E MÉTODO DE FABRICAÇÃO DE DISPOSITIVO MICROFLUÍDICO PCT/BR2017/000052 - Universidade Estadual de Campinas (UNICAMP) . Ronaldo Censi Faria; Carolina Venturini Uliana; André Santiago Afonso - May 2017, 15