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Action of oxytocin in the process of osteoblast differentiation in senescent rats

Grant number: 11/15501-0
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): September 01, 2012
Effective date (End): July 31, 2014
Field of knowledge:Biological Sciences - Physiology - Physiology of Organs and Systems
Principal Investigator:Rita Cássia Menegati Dornelles
Grantee:Leandro Figueiredo dos Santos
Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil


Study realized in our laboratory showed that oxytocin promoted increased formation and inhibited alveolar bone resorption in rats with 24 months when compared with the same age group who did not receive the hormone. However, in rats of 12 months the treatment with oxytocin did not induce significant changes in the process of bone regeneration. Through these results, we question whether the osteoblasts of rats of different ages are able to maintain the ability to synthesize oxytocin and respond to their stimulus, and if there autocrine pathway between osteoblasts and oxytocin. Therefore, it is important investigate the cellular mechanisms involved in bone formation in response to oxytocin, understand the mechanisms involved in increased bone formation in senile organisms, in a period characterized by the estrous acyclicity (estropause - 24 months) compared with adult organisms with regular estrous cycle (12 months). This study will evaluate the influence of age and the action of oxytocin associated with classical inducers during differentiation in the osteogenic lineage of stem cells from bone marrow of Wistar rats with 12 and 24 months of age. The mesenchymal stem cells will be collected of the following groups: 1 = Control/12 months (proliferation medium); 2 = Control + oxytocin/12 months (proliferation medium + oxytocin); 3 = Medium osteogenic/12 months (ascorbic acid + dexamethasone + beta-glycerophosphate); 4 = Medium osteogenic oxytocin/12 months (ascorbic acid + beta-glycerophosphate + dexamethasone + oxytocin); Control/24 = 5 months (proliferation medium); 6 = Control + oxytocin/24 months (proliferation medium + oxytocin); 7 = Medium osteogenic/24 months (ascorbic acid + dexamethasone + beta-glycerophosphate) and 8 = Medium osteogenic oxytocin/24 + months (ascorbic acid + beta-glycerophosphate + dexamethasone + oxytocin). Will be performed to analyze cell proliferation, measuring alkaline phosphatase activity, detection and quantification of mineralization of extracellular matrix gene expression of oxytocin, oxytocin receptor (OTR) and matrix proteins collagen and non-collagen in cultured osteoblasts (Extraction and quantification of total RNA and reverse transcription, quantitative PCR), and is determined by ELISA the concentration of prostaglandin E2.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SANTOS, LEANDRO FIGUEIREDO; SINGULANI, MONIQUE PATRICIO; STRINGHETTA-GARCIA, CAMILA TAMI; PENHA OLIVEIRA, SANDRA HELENA; CHAVES-NETO, ANTONIO HERNANDES; MENEGATI DORNELLES, RITA CASSIA. Oxytocin effects on osteoblastic differentiation of Bone Marrow Mesenchymal Stem Cells from adult and aging female Wistar rats. Experimental Gerontology, v. 113, p. 58-63, . (11/15501-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SANTOS, Leandro Figueiredo dos. Atuação da ocitocina no processo de diferenciação osteoblástica de ratas senis. 2014. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba Araçatuba.

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