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Effects of a probiotic (Enterococcus faecium CRL 183 and Bifidobacterium longum ATCC 15707) fermented soy product on the development of chemically induced colitis in rats

Grant number: 12/07706-3
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): August 01, 2012
Effective date (End): February 28, 2014
Field of knowledge:Agronomical Sciences - Food Science and Technology - Food Science
Principal Investigator:Daniela Cardoso Umbelino Cavallini
Grantee:Larissa Sbaglia Celiberto
Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Some strains of probiotic microorganism have the potential to assist in relieving the symptoms of inflammatory bowel disease (IBD). The aim of this study is to determine the effects of daily ingestion of a product based on an aqueous extract of soybean, fermented by Enterococcus faecium CRL 183 and Lactobacillus helveticus 416 with the addition of Bifidobacterium longum ATCC 15707, on chemically induced colitis in rats and to investigate the possible associated mechanisms. Colitis will be induced chemically by 4% dextran sulfate sodium (DSS), dissolved in the drinking water supplied to the animal, which will be ingested daily for a period of seven days. The animals will then be assigned randomly to four groups (n = 10): Group C: healthy animals that do not receive the products under study; Group CL: animals with chemically induced colitis that do not receive the products under study; Group CLF: animals with chemically induced colitis that receive the fermented product; Group PLC: animals with chemically induced colitis that receive the non-fermented product (placebo). Throughout the trial period of 30 days the following parameters will be monitored: body weight of animals, daily water and food intake, consistency and presence of fecal occult blood, fecal microbiota composition, gastrointestinal survival of probiotic microorganisms and concentrations of polyamines and SCFA in the faeces. At the end of the treatment period the animals will be euthanized and the intestine removed to perform histological analysis of the colon and rectum, and determine the concentration of cytokines in the colon.

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Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
CELIBERTO, Larissa Sbaglia. Efeito de uma bebida probiótica (Enterococcus faecium CRL 183 e Bifidobacterium longum ATCC 15707) à base de extrato aquoso de soja no desenvolvimento de colite quimicamente induzida em ratos. 2014. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Farmacêuticas. Araraquara Araraquara.

Please report errors in scientific publications list by writing to: gei-bv@fapesp.br.