Analysis of cellular mechanisms of acute injuries in the central and peripheral nervous system using novel imaging approaches

• Grant number: 12/13514-0
• Support type: Scholarships abroad - Research Internship - Master's degree
• Effective date (Start): November 19, 2012
• Effective date (End): March 18, 2013
• Field of knowledge: Biological Sciences - Morphology - Anatomy
• Principal researcher: Alexandre Leite Rodrigues de Oliveira
• Grantee: Luciana Politti Cartarozzi
• Supervisor abroad: Frank Kirchhoff
• Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Research place: Saarland University, Saarbrücken, Germany
• Associated to the scholarship: 11/02889-0 - Use of fibrin sealant combined with mesenchymal stem cells in the repair of peripheral nerves through tubulization technique, BP.MS

Abstract

For analysis of the progression of regeneration in the central and peripheral nervous system usually are used techniques from histological sections that provide a two-dimensional image of the regenerating axons, showing only fragments, and may thus lead to misinterpretations. In order to overcome this obstacle, the tetrahydrofuran (THF)-based clearing technique was proposed. Through this technique, the brain stem and spinal cords of rodents become transparent allowing the deep-tissue imaging of axons. Since this technique is standardized only for spinal cord, this paper proposes the establishment of the technique for peripheral nerves. For this, we will use transgenic mice that express fluorescent proteins in neurons, astrocytes and microglia as well as double and triple hybrids. Microscopic analysis, by 2P-LSM will include the observation of cleared tissues (spinal cord and sciatic nerves) from transgenic mice before and after injury. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BISCOLA, NATALIA PERUSSI; CARTAROZZI, LUCIANA POLITTI; ULIAN-BENITEZ, SUZANA; BARBIZAN, ROBERTA; CASTRO, MATEUS VIDIGAL; SPEJO, ALINE BARROSO; FERREIRA, JR., RUI SEABRA; BARRAVIERA, BENEDITO; RODRIGUES OLIVEIRA, ALEXANDRE LEITE. Multiple uses of fibrin sealant for nervous system treatment following injury and disease. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 23, n. 1 MAR 14 2017. Web of Science Citations: 19.