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Characterization of wound healing in mice subjected to Type 1 Diabetes Mellitus, on oral administration of linoleic acid

Grant number: 12/11967-7
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): August 01, 2012
Effective date (End): March 31, 2013
Field of knowledge:Health Sciences - Nutrition
Principal Investigator:Hosana Gomes Rodrigues
Grantee:Ana Sayuri Yamagata
Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil


In healing, an important process of defense, the effects of linoleic acid intake by diabetic animals will be investigated. The skin is one of the first natural barriers between the body and antigens, and therefore its injury should be quickly corrected. The dermal wound repair corresponds to a sequence of processes comprising respectively inflammation, granulation tissue formation, and tissue remodeling. As recently demonstrated by our group, intake of unsaturated fatty acids modulates inflammation by regulating the production of cytokines and growth factors. Apparently, this explains the use of these lipids in ulcer and skin damage, although this modulation is not yet well understood. Thus, the issue deserves further investigation. In this study, mice C57Black / 6 will be divided into 4 groups: control (C), diabetic (D), control supplemented with linoleic acid (CL), and diabetic supplemented with linoleic acid (DL). Diabetes will be induced by streptozotocin (65 mg/kg) intravenously. Will be considered diabetic animals with blood glucose levels higher than 250 mg / dL. After confirmation of type 1 diabetes, will begin the gavage. The animals will receive linoleic acid (0.44 g / kg body weight) three times a week. Five days after initiation of gavage, will be held back in the wounds of the animals. Analysis of scar tissue will take place in times 0, 1, 3, 5, 10, and 14 days. We will analyze macroscopic wound closure, cell phenotyping by flow cytometry (FACS) and ELISA (enzyme-linked immunosorbent assay) to quantify cytokines. Data will be analyzed by One-way ANOVA and Tukey post-test.(AU)

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