Synthesis of magnetic nanoparticles modified with biomimetic polymer for Penicillin-G

• Grant number: 12/14181-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2012
• End date: November 30, 2014
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Maria Del Pilar Taboada Sotomayor
• Grantee: Rafael Rovatti Pupin
• Host Institution: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

The pharmaceutical industry has evolved considerably in recent decades, producing thousands of different drugs and thus contributing to improved health. Among the most important drugs is the ²-lactam antibiotics (such as benzyl penicillin or simply penicillin G) used as antimicrobial agents.However, these drugs have been discarded in the environment, mainly polluted river water. Another problem is the fact that this drug could be residues in milk, as is the penicillin antibiotic most administered to dairy cows for treating mastitis. These penicillin residues pose risks to health due to the high number of people who are allergic to the medicine.Thus, this project aims to develop analytical systems through selective molecularly imprinted polymers (MIP) immobilized on magnetic nanoparticles to detect traces of penicillin present in complex matrices such as honey and milk. MIP, also called biomimetic polymers by mimicking affinity interactions such as the antibody-antigen, to be modified on the magnetic nanoparticles will act selectively on the extraction of the analyte of interest (penicillin G), while the magnetic nanoparticles allow the selective separation of analyte from complex matrices to be evaluated, thereby increasing the detectability and reliability analysis, which may be made by electrochemical or chromatographic techniques. The magnetic nanoparticles, the order of 20 nm will be synthesized by the method of co-precipitation and then be modified with the MIP using the bulk method.Once synthesized the magneto-MIP, the efficiency of retention and selectivity of the analyte will be studied by binding isotherms, kinetics of adsorption time, optimization of pH and solvent extraction, then the materials will be evaluated in samples enriched penicillin G, such as milk and honey fresh and commercial, which will be analyzed by chromatography and by electroanalytical techniques in order to obtain the most sensitive, faster and more cost methodology for this type of analysis. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PUPIN, RAFAEL ROVATTI; FOGUEL, MARCOS VINICIUS; GONCALVES, LUIS MOREIRA; SOTOMAYOR, MARIA DEL PILAR T.. Magnetic molecularly imprinted polymers obtained by photopolymerization for selective recognition of penicillin G. Journal of Applied Polymer Science, v. 137, n. 13, APR 5 2020. (18/14425-7, 16/06926-0, 12/14181-4)