Blood biomarkers for Alzheimer's Disease: evaluation of ADAM10 gene expression and micro-RNAs

• Grant number: 12/08654-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): October 01, 2012
• Effective date (End): February 29, 2016
• Field of knowledge: Biological Sciences - Physiology - General Physiology
• Principal Investigator: Márcia Regina Cominetti
• Grantee: Patricia Regina Manzine Moralles
• Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Associated scholarship(s): 13/19521-0 - Laboratory techniques for the analysis of synaptic ADAM10 expression in Alzheimer's Disease, BE.EP.DR

Abstract

Alzheimer's disease (AD) stands out as the leading cause of dementia in the elderly. Molecular biomarkers of direct or indirect relation to the physiopathology of the disease have been identified. Among them, some studies show a decrease of platelet ADAM10 protein in AD patients, as well as the deregulation of microRNAs (miRNAs) related to the gene expression of molecules involved in the pathophysiology of this disease. This project is a comparison study, based on the assumptions of quantitative research. The main objective is to verify and compare the gene expression of ADAM10 and miRNAs in subjects with mild cognitive impairment (MCI) and dementia of AD, with elderly people without cognitive impairments. It will be held in the city of San Carlos, headquarters of the Federal University of São Carlos (UFSCar). Biological samples of research subjects will be collected, analyzed and then stored in a biorepository. The whole blood of subjects will be studied by qPCR techniques, using differential expression in comparison with healthy subjects. Statistical tests of association, regression and diagnostic accuracy will be carried out. The results could contribute to the deployment of tools that work in the early diagnosis of AD, such as the construction of a diagnosis kit for measuring plasma levels of ADAM10 and specific miRNAs between healthy subjects, with MCI and AD. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MANZINE, PATRICIA REGINA; SOUZA, MATHEUS DA SILVA; COMINETTI, MARCIA REGINA. BACE1 levels are increased in plasma of Alzheimer's disease patients compared with matched cognitively healthy controls. Personalized Medicine, v. 13, n. 6, p. 531-540, NOV 2016. (14/06580-1, 13/06879-4, 12/08654-7)

MANZINE, PATRICIA R.; PELUCCHI, SILVIA; HORST, MARIA A.; VALE, FRANCISCO A. C.; PAVARINI, SOFIA C. I.; AUDANO, MATTEO; MITRO, NICO; DI LUCA, MONICA; MARCELLO, ELENA; COMINETTI, MARCIA R.. microRNA 221 Targets ADAM10 mRNA and is Downregulated in Alzheimer's Disease. JOURNAL OF ALZHEIMER'S DISEASE, v. 61, n. 1, p. 113-123, 2018. (13/06879-4, 12/08654-7)

MANZINE, PATRICIA REGINA; MARCELLO, ELENA; BORRONI, BARBARA; KAMPHUIS, WILLEM; HOL, ELLY; PADOVANI, ALESSANDRO; NASCIMENTO, CARLA CRISPIM; BUENO, PATRICIA DE GODOY; CARVALHO VALE, FRANCISCO ASSIS; PAVARINI, SOFIA CRISTINA LOST; et al. ADAM10 gene expression in the blood cells of Alzheimer's disease patients and mild cognitive impairment subjects. BIOMARKERS, v. 20, n. 3, p. 196-201, 2015. (13/06879-4, 10/09497-7, 12/08654-7)

FERNANDES MARQUES BIANCO, OTAVIO AUGUSTO; MANZINE, PATRICIA REGINA; CRISPIM NASCIMENTO, CARLA MANUELA; CARVALHO VALE, FRANCISCO ASSIS; IOST PAVARINI, SOFIA CRISTINA; COMINETTI, MARCIA REGINA. Serotoninergic antidepressants positively affect platelet ADAM10 expression in patients with Alzheimer's disease. INTERNATIONAL PSYCHOGERIATRICS, v. 28, n. 6, p. 939-944, JUN 2016. (10/09497-7, 12/01936-7, 14/21066-2, 13/06879-4, 12/08654-7)