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Coffee cell suspension exposed to nickel

Grant number: 11/22245-0
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): May 01, 2012
Effective date (End): December 31, 2012
Field of knowledge:Agronomical Sciences - Agronomy
Principal Investigator:José Laércio Favarin
Grantee:Tiago Tezotto
Supervisor abroad: Joseph Carmine Polacco
Home Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
Local de pesquisa : University of Missouri, Columbia (UM), United States  
Associated to the scholarship:11/05928-6 - Nickel nutrition in coffee plant and its relationship with nitrogen metabolism and senescence, BP.DR


Nickel (Ni) has been regarded as essential micronutrient for plants to be a constituent of the active site of the enzyme urease, which hydrolyzes urea to ammonia and bicarbonate. The urease protein is abundant in seeds of some plant species and is also found at low levels in vegetative tissues of many, if not all, plants. A deficiency of this enzyme leads to accumulation of urea and subsequent formation of necrotic lesions on leaves. This dependence of urease in relation to this element indicates that Ni is an essential micronutrient for at least the conditions under which urea is the main source of N, is applied as fertilizer or released during the mobilization of N stored mainly as arginine. Ni can be remobilized from plant organs, which can a desirable feature when there is demand for urease activity, which is particularly the case for plants fertilized with urea. In thesis project is planned to study the role of Ni in plants and this request is to study this element in coffee suspension cells display effect of Ni in the activation and activity of urease protein grown with different forms of nitrogen (N -NO3-, NH4 +-N and urea-N). The supervisor of the laboratory that will receive us in the United States, Dr. Joseph Polacco, is the world's leading authority on the metabolism of urea / urease in plants and received us with great interest, because recent data obtained show that coffee is a culture completely different from others that he worked so far. In fact, almost everything that is known in x Ni urease was obtained from soybeans. The trip to his lab not only allow the development of the research itself, but a great enrichment in my education, and seal a collaboration that began recently, with the submission of a revision to an Elsevier's Encyclopedia (Encyclopedia of Metalloproteins) and a review for Plant Science. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
TEZOTTO, TIAGO; RIBEIRO SOUZA, SARAH CAROLINE; MIHAIL, JEANNE; FAVARIN, JOSE LAERCIO; MAZZAFERA, PAULO; BILYEU, KRISTIN; POLACCO, JOE CARMINE. Deletion of the single UreG urease activation gene in soybean NIL lines: characterization and pleiotropic effects. THEORETICAL AND EXPERIMENTAL PLANT PHYSIOLOGY, v. 28, n. 3, p. 307-320, SEP 2016. Web of Science Citations: 3.

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