In vitro studies of the potencial of oxidative stress and immunotoxic potencial of basic red 51 and basic yellow 57 in keratinocytes

• Grant number: 12/05961-6
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Effective date (Start): November 01, 2012
• Effective date (End): September 30, 2013
• Field of knowledge: Health Sciences - Pharmacy - Toxicological Analysis
• Principal Investigator: Danielle Palma de Oliveira
• Grantee: Thalita Boldrin Zanoni
• Supervisor: Geja J. Hageman
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Research place: Maastricht University, Maastricht (UM), Netherlands
• Associated to the scholarship: 10/07116-6 - Profile evaluation of citotoxicity, mutagenicty and genotoxicity of the dyes Basic Red 51, Basic Yellow 57 and P-Phenylenodiamine used in hair dye on skin cells., BP.DR

Abstract

Nowdays,the use of sinthetic hair dyes has increased. Many of these dyes belong to the class of azo dyes (-N=N-). It is known that aromatic amines can be generated after the breakage of the azo group leading to toxic compounds that may be harmfull to human beings and animals.According to the literature, some aromatic amines can be absorbed by the skin leading to mutagenic, carcinogenicity and alergic reactions. For that purpose, considering the intense use of these dyes, we suggest that it is necessary to better evaluate the toxic properties of these commercial dyes. This project proposes the analisys of two toxicological pathways that will investigate the potential of inducing DNA adducts on keratinocytes as well as the investigation of the imunotoxicity of the hair dyes Basic Red 51 and Basic Yellow 57. We expect that the results of this work will lead to new findings of toxicologycal data that will contribute to the development of more safe commercial products. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ZANONI, THALITA B.; HUDARI, FELIPE; MUNNIA, ARMELLE; PELUSO, MARCO; GODSCHALK, ROGER W.; ZANONI, MARIA VALNICE B.; DEN HARTOG, GERTJAN J. M.; BAST, AALT; BARROS, SILVIA B. M.; MARIA-ENGLER, SILVYA S.; et al. The oxidation of p-phenylenediamine, an ingredient used for permanent hair dyeing purposes, leads to the formation of hydroxyl radicals: Oxidative stress and DNA damage in human immortalized keratinocytes. Toxicology Letters, v. 239, n. 3, p. 194-204, DEC 15 2015. (08/10449-7, 10/07116-6, 12/05961-6)