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Effect of breed on biochemical traits influencing beef Longissimus tenderness

Grant number: 12/06618-3
Support Opportunities:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): October 15, 2012
Effective date (End): July 14, 2013
Field of knowledge:Agronomical Sciences - Animal Husbandry - Animal Production
Principal Investigator:Telma Teresinha Berchielli
Grantee:Josiane Fonseca Lage
Supervisor: Steven D. Shackelford
Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
Research place: U.S. Meat Animal Research Center (MARC), United States  
Associated to the scholarship:09/18431-2 - Crude glycerine coming from the agribusiness of biodiesel in the diet of steers containing high or low level of starch in different ratios of roughage and concentrate, BP.DR


Samples will be obtained from on-going beef breed evaluation studies at the U.S. Meat Animal Research Center. Breeds represented will include progeny of the 17 most common beef breeds in the United States. Steers (n = 1,200) will be grain-finished and harvested in commercial packing plants. Following the packing plants' routine grading procedures (i.e., at 24 to 96 h postmortem), longissimus (LM) steaks will be obtained from the anterior end of the strip loin (13th rib region). Steaks will be transported to USMARC and aged (1pC) until 14 d postmortem. Fresh (never frozen) steaks will be cooked (71pC) and slice shear force (Shackelford et al., 1999) will be measured. The remnants of the slice shear force sample (cooked LM) will be frozen (-20pC) and stored for subsequent measurement of biochemical traits influencing beef longissimus tenderness. Subsequently, 6 blocks (2 mm × 2 mm × 3 mm; 1 or 2 blocks from the center of each per half-slice remnant) will be obtained parallel to the muscle fiber orientation and 6 fibers will be teased from each block, and sarcomere length will be determined for each fiber by laser diffraction using the procedure of Cross et al. (1981). Thus, each observation for sarcomere length will represent the mean of 36 fibers. The remainder of the trimmed half-slice remnants will be diced and powdered with liquid nitrogen and postmortem proteolysis will be assessed by measuring the extent of degradation of desmin by western blotting as described by Wheeler et al. (2002). (AU)

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