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Development of analytical procedure for the determination of antibiotics in water using microextraction techniques and sequential injection chromatography

Grant number: 12/14950-8
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): March 01, 2013
Effective date (End): August 31, 2013
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
Principal researcher:Fábio Rodrigo Piovezani Rocha
Grantee:Alex Domingues Batista
Supervisor abroad: Petr Solich
Home Institution: Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
Research place: Charles University in Prague (CU), Czech Republic  
Associated to the scholarship:11/06437-6 - Flow analysis systems applied to the determination of emerging pollutants, BP.DR


Antibiotics are widely used as antimicrobial agents in animals and humans. Many of these molecules are not completely metabolized by the body and they are inserted in the environment through the generated waste. These compounds are found in soil and water and they can promote the resistance of antibiotic in microorganisms, and they are toxic to certain organisms. The aim of this project is to develop procedures for sample preparation hyphenated with sequential injection chromatography for the determination of ²-lactam antibiotics in water. For the development of analytical procedures, we intend to employ procedures for solid phase or liquid-liquid microextraction in order to minimize the sample and solvent consumption and waste generation, economic and environmental important factors. The hyphenation with sequential injection chromatography systems may enable the development of faster, cheaper and environmentally friendly procedures for the determination of antibiotics in water due to the possibility of managing mobile phases and reagents. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BATISTA, ALEX D.; CHOCHOLOUS, PETR; SATINSKY, DALIBOR; SOLICH, PETR; ROCHA, FABIO R. P.. On-line hyphenation of solid-phase extraction to chromatographic separation of sulfonamides with fused-core columns in sequential injection chromatography. Talanta, v. 133, n. SI, p. 142-149, . (11/06437-6, 12/14950-8)

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