Study of hypoxya and oxidative stress markers at different life period of diabetic rats

• Grant number: 12/16084-6
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: February 01, 2013
• End date: March 31, 2013
• Field of knowledge: Health Sciences - Medicine - Maternal and Child Health
• Principal Investigator: Débora Cristina Damasceno
• Grantee: Bruna Dallaqua
• Supervisor: Leslie Myatt
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Institution abroad: University of Texas Health Science Center at Houston (UTHealth), United States
• Associated to the scholarship: 11/16241-1 - STUDY OF HYPOXYA AND OXIDATIVE STRESS AT DIFFERENT LIFE PERIOD OF THE RATS AND THEIR OFFSPRING IN THE DIABETIC STATUS, BP.DR

Abstract

There is evidence that hyperglycemia induces increased production of reactive oxygen species (ROS). The main source intracellular of ROS is the mitochondria and alterations associated to the mitochondrial phenotype in the diabetes have been widely described. To promote the mitochondrial biogenesis, studies show that peroxisome proliferator-activated receptor coactivator 1 alfa (PGC-1alfa) plays an important role. In addition, this protein is widely distributed in tissues and plays a key role in regulating cellular development and homeostasis of oxygen (O2). It has been shown that the mitochondrial biogenesis induced by PGC-1alpha increases O2 consumption, leading to decreased intracellular oxygen availability to HIF-hydroxylase enzymes, stabilizing the HIF-1alpha. The hypoxia-inducible factors (HIF) are among the transcription proteins more defined and identified as being regulated by intracellular redox state. HIF-1alpha is the main regulator of the O2 homeostase. In low concentrations of O2, there is activation of other factors to increase the liberation of O2 for the cells, facilitating the production of ATP. With this, our research group aims to analyze hypoxia and oxidative stress markers in liver of streptozotocin-induced mild diabetic rats to explain the mechanisms responsible for changes caused by diabetes and /or hypoxia. This project is being conducted in Brazil but a part of the methodology will be learning in the U.S., because the supervisor of exterior has an acknowledged international expertise in the kind of research. In addition, the stage will bring important contribution to the applicant, advisor, graduate program, other teachers and students involved in this research project. (AU)