|Support type:||Scholarships in Brazil - Scientific Initiation|
|Effective date (Start):||October 01, 2012|
|Effective date (End):||December 31, 2013|
|Field of knowledge:||Biological Sciences - Biochemistry - Molecular Biology|
|Principal Investigator:||Tatiana Maria de Souza Moreira|
|Grantee:||Cristina Carvalho Barbosa|
|Home Institution:||Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil|
An important class of secundary metabolites in plant is the pentacyclic triterpenes. These coumpounds have various functions such as insecticidal, antimicrobial and are widely used in pharmaceutical, food and cosmetics. The pentacyclic triterpenes are formed by the oxidosqualene cyclization reaction by oxidosqualene cyclase enzymes (OSC) giving molecules of primary metabolism present in the membrane of prokaryotes or eukaryotes or molecules with hormonal functions. At this moment there is a lack in databases concerning the number of gene sequences that allows the knowledge of the variety of gene and enzymes (OSC). So, the aim of the present project is to contribute to the knowledge of biological diversity through the cloning of genes that encode oxisqualene cyclases in the leaves of M. ilicifolia. After mRNA extraction from the leaves of M. ilicifolia the cDNA will be synthetized, which will be used for the amplification of the interested gene fragments using primers with conserved regions of OSCs. Subsequent to the cloning of the fragments and sequencing, the information obtained will be used to design specific oligonucleotide primers for the extension of the 5' and 3' ends in order to efficiently clone the complete fragment of the genes of oxidosqualene cyclases.