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Development of Real Time RT-PCR techniques for arbovirus that cause human disease and nucleotide sequencing identification of them

Grant number: 12/02836-6
Support type:Scholarships in Brazil - Master
Effective date (Start): November 01, 2012
Effective date (End): August 31, 2014
Field of knowledge:Health Sciences - Medicine
Principal Investigator:Luiz Tadeu Moraes Figueiredo
Grantee:Marilia Farignoli Romeiro
Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The arboviruses related families Flaviviridae, Togaviridae, and Bunyaviridae include serious public health problems in tropical and subtropical countries. These arboviruses generally misdiagnosed, concerning clinical manifestations ranging from asymptomatic infection, acute febrile disease viral hemorrhagic fever and encephalitis.These arboviruses are maintained in nature through cycles that comprise natural hosts (amplifiers) like birds, mammals and some reptiles, and in vectors of the genus Aedes sp. mosquitoes, Culex sp., Sabethes sp. and Psorophora sp., sometimes they can transmit these viruses to accidental hosts like humans, horses and other mammals. The methodologies used in the diagnosis of arboviral infection are based on serological tests such as ELISA, and virological tests. With the revolution of molecular techniques, the polymerase chain reaction (PCR) have been widely used in the diagnosis of arboviral infections. The technique of real time RT-PCR brings advantages over conventional PCR technique highlighting the high sensitivity and specificity, and quantification of viral load from samples. The present study aims to develop a real time RT-PCR based on SYBR Green detection system to diagnosis human arboviral diseases and investigates, by sequencing analisys, the presence of Flavivirus, Alphavirus, Phlebovirus and Orthobunyavirus in human samples.

Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ROMEIRO, MARILIA FARIGNOLI; DE SOUZA, WILLIAM MARCIEL; TOLARDO, ALINE LAVADO; VIEIRA, LUIZ CARLOS; HENRIQUES, DYANA ALVES; DE ARAUJO, JANSEN; HASSEGAWA SIQUEIRA, CARLOS EDUARDO; COLOMBO, TATIANA ELIAS; AQUINO, VICTOR HUGO; LOPES DA FONSECA, BENEDITO ANTONIO; DE MORAIS BRONZONI, ROBERTA VIEIRA; NOGUEIRA, MAURICIO LACERDA; DURIGON, EDISON LUIZ; MORAES FIGUEIREDO, LUIZ TADEU. A real-time RT-PCR for rapid detection and quantification of mosquito-borne alphaviruses. ARCHIVES OF VIROLOGY, v. 161, n. 11, p. 3171-3177, NOV 2016. Web of Science Citations: 1.
ALINE LAVADO TOLARDO; WILLIAM MARCIEL DE SOUZA; MARILIA FARIGNOLI ROMEIRO; LUIZ CARLOS VIEIRA; LUCIANO KLEBER DE SOUZA LUNA; DYANA ALVES HENRIQUES; JANSEN DE ARAUJO; CARLOS EDUARDO HASSEGAWA SIQUEIRA; TATIANA ELIAS COLOMBO; VICTOR HUGO AQUINO; BENEDITO ANTONIO LOPES DA FONSECA; ROBERTA VIEIRA DE MORAIS BRONZONI; MAURÍCIO LACERDA NOGUEIRA; EDISON LUIZ DURIGON; LUIZ TADEU MORAES FIGUEIREDO. A real-time reverse transcriptase polymerase chain reaction for detection and quantification of Vesiculovirus. Memórias do Instituto Oswaldo Cruz, v. 111, n. 6, p. 385-390, Jun. 2016. Web of Science Citations: 2.
ROMEIRO, MARILIA FARIGNOLI; DE SOUZA, WILLIAM MARCIEL; TOLARDO, ALINE LAVADO; VIEIRA, LUIZ CARLOS; COLOMBO, TATIANA ELIAS; AQUINO, VICTOR HUGO; NOGUEIRA, MAURICIO LACERDA; MORAES FIGUEIREDO, LUIZ TADEU. Evaluation and optimization of SYBR Green real-time reverse transcription polymerase chain reaction as a tool for diagnosis of the Flavivirus genus in Brazil. Revista da Sociedade Brasileira de Medicina Tropical, v. 49, n. 3, p. 279-285, MAY-JUN 2016. Web of Science Citations: 2.
MOTTA MAIA, FELIPE GONCALVES; CHAVEZ, JULIANA HELENA; DE SOUZA, WILLIAM MARCIEL; ROMEIRO, MARILIA FARIGNOLI; DE CASTRO-JORGE, LUIZA ANTUNES; LOPES DA FONSECA, BENEDITO ANTONIO; MORAES FIGUEIREDO, LUIZ TADEU. Infection with Saint Louis encephalitis virus in the city of Ribeirao Preto, Brazil: report of one case. INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, v. 26, p. 96-97, SEP 2014. Web of Science Citations: 6.
SILVA, JAQUELINE RAYMONDI; ROMEIRO, MARILIA FARIGNOLI; DE SOUZA, WILLIAM MARCIEL; MUNHOZ, THIAGO DEMARCHI; BORGES, GUSTAVO PUIA; BRIOSCHI SOARES, OTAVIO AUGUSTO; COELHO DE CAMPOS, CARLOS HENRIQUE; MACHADO, ROSANGELA ZACARIAS; CRISTINY RODRIGUES SILVA, MARIA LUANA; MAIA FARIA, JOICE LARA; CHAVEZ, JULIANA HELENA; MORAES FIGUEIREDO, LUIZ TADEU. A Saint Louis encephalitis and Rocio virus serosurvey in Brazilian horses. Revista da Sociedade Brasileira de Medicina Tropical, v. 47, n. 4, p. 414-417, JUL-AUG 2014. Web of Science Citations: 11.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.