Toxicological and pharmacological in vitro and in vivo prospection of oil resins, leaf extracts and isolated compounds from Copaifera oblongifolia Mart. ex Hayne and C. duckei Dwyer

• Grant number: 12/09727-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): November 01, 2012
• Effective date (End): August 31, 2016
• Field of knowledge: Health Sciences - Pharmacy - Pharmacognosy
• Principal Investigator: Jairo Kenupp Bastos
• Grantee: Marivane Lemos
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The use of medicinal plants is a practice that follows the evolution of humanity. Despite the copaiba oleoresin is widely used by the Brazilian population and there are several studies that confirm these applications, there is still need for further chemical, biological and clinical toxicology mechanisms, ensuring the effectiveness, safety and quality for the future development of phytotherapic. In addition, there are few studies on the chemical composition of leaves of Copaifera, which is little explored, given that the marketing of the oleoresin of the trunk moves about R$ 1.5 billion in 2007. From all indications medicinal, its anti-inflammatory, antimicrobial, healing of wounds and ulcers are the most frequently reported. A stomach ulcer and pain accompanied by inflammation, both acute and chronic affect much of the world population. The genesis of chronic inflammatory diseases are multifactorial, and that chronic pain has not yet fully understood its pathophysiological mechanism. Affecting approximately 20 million people worldwide, causing personal burden (pain and functional disability) and inestimable economic losses. Treatments should not produce large risks, but have many side effects, and often do not reach the ideal pharmacological effect. Already a stomach ulcer affects a large part of the world population, reaching approximately 10% of the economically active population. Its pathophysiology is complex, caused by an imbalance between aggressive and protective factors present in the stomach, characterized by the formation of lesions and necrosis of the gastric tissue. Within this context, this project seeks to assess and understand the mechanisms involved in the processes of cytoprotection and healing of gastric ulcers, the anti-inflammatory drugs and analgesics, commonly assigned and previously described for the copaibas oleoresin. There are few studies to C. oblongifolia Mart. ex Hayne and C. duckei Dwyer. So, be obtained from leaves and oleoresin, which will be submitted to the fractionation and isolation of the major compounds using different chromatographic methods: liquid-liquid partition, open columns, sephadex gel, HSCCC, HPLC, among others. The metabolites are identified using spectrometric and spectroscopic techniques. For the pharmacological tests with oleoresin and extracts, fractions and isolated metabolites, the study of nociception will be performed by methods widely described to evaluate the threshold for nociception, hyperalgesia (von Frey) and chronic painful process (brachial plexus avulsion). Studies on the potential antiinflammatory effect will be realized through the model of paw oedema and pleurisy. Already the evaluation models the gastroprotection are based on induced models of acute ulcer, chronic ulcers and gastric parameters evaluation. In the three pharmacological approaches, this study investigates the possible mechanisms involved in pharmacological effects and the data obtained, besides contributing to the knowledge of the mechanisms of action of the plants of the genus Copaifera, will provide subsidies for further studies that can serve to the development a new phytotherapic in Brazil.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MOTTA, ERICK V. S.; LEMOS, MARIVANE; COSTA, JULIANA C.; BANDERO-FILHO, VILMAR C.; SASSE, ASTRID; SHERIDAN, HELEN; BASTOS, JAIRO K.. Galloylquinic acid derivatives from Copaifera langsdorffii leaves display gastroprotective activity. Chemico-Biological Interactions, v. 261, p. 145-155, JAN 5 2017. (11/13630-7, 12/09727-8, 12/01336-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

LEMOS, Marivane. Toxicological and pharmacological prospection in vitro and in vivo oil resins, leaf extracts and isolated compounds from Copaifera oblongifolia Mart. ex Hayne and C. duckei Dwyer. 2016. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) Ribeirão Preto.