|Support type:||Scholarships in Brazil - Master|
|Effective date (Start):||October 01, 2012|
|Effective date (End):||April 30, 2014|
|Field of knowledge:||Biological Sciences - Morphology - Embryology|
|Principal Investigator:||Wilma de Grava Kempinas|
|Grantee:||Josiane de Lima Rosa|
|Home Institution:||Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil|
Interferons (IFNs) are small proteins known for their antiproliferative and immunoregulatory activities and their crucial involvement in cellular antiviral action. The type I IFNs include IFN alpha, beta and omega that are used therapeutically in the treatment of various diseases such as metastatic melanoma, acuminata condyloma, Kaposi's sarcoma and hepatitis B and C. Studies indicate that IFN may be an endocrine disruptor with direct or indirect action on the male gonads. It's known that the drug can interfere on physiological function of the endothelial barrier and possibly in epithelial cells. The blood-testis barrier (HT) and blood-epididymal (HE) consist in tight junctions formed by adjacent epithelial cells of the seminiferous tubule or of the epididymal duct, respectively. These tight junctions are composed of several proteins, among them claudines (CLDNs), and the expression of different claudines along the seminiferous tubule and epididymal duct may represent a key element in the regulation changes of the intraluminal environment, that plays an essential role in reproductive functions. The aim of this study will be to investigate male reproductive parameters, with emphasis on the effects of INF alpha on molecular aspects of the barriers HT and HE. For this purpose, adult male Wistar rats (90 days) will be treated, subcutaneously, for 30 days, with IFN alpha and then evaluated for sperm parameters, hormone levels (luteinizing hormone (LH), follicle stimulating hormone (FSH) and testosterone), and histological and morphometric analysis of testis and epididymis. Sperm quality will also be assessed by in utero artificial insemination . Molecular aspects of the HT and HE barriers will be evaluated by immunohistochemistry using marking of CLDN 3 and 11 in the testis and CLDN 1 and 3 in the epididymis. Besides the preparation of qualified human resources in Biology and Toxicology of Reproduction, we expect to acquire new knowledgement on the effects of interferon alpha on reproductive parameters of male rats, disseminating the results in publications in international journals, as well in communications in scientific events of this area. Moreover, to establish collaboration with internacional researchers in the area do Biology and Toxicology of Reproduction.