Cytokine gene expression from cocultures of lymphocytes and dendritic cells derived from monocytes of HIV-infected children and adolescents

• Grant number: 12/06525-5
• Support Opportunities: Scholarships in Brazil - Master
• Start date: November 01, 2012
• End date: August 31, 2014
• Field of knowledge: Health Sciences - Medicine - Maternal and Child Health
• Principal Investigator: Maria Marluce dos Santos Vilela
• Grantee: Jéssica Santana Bernachi
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The aim of this study is to characterize in HIV-infected children and adolescents the response in cocultures of monocyte derived dendritic cells (MDDC) and lymphocytes to inactivated HIV particles. The dendritic cells will be evaluated by flow cytometry for expression of CD80, CD83, CD86, CD40 and HLA-DR and by PCR-array followed by Real Time PCR for cytokines mRNA expression of IFN-gama, IFN-alfa, TNF-alfa, TGF-beta 1, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12 e IL-15. The results from patients with viral load> 1000 copies/mL and with undetectable viral load will be compared using statistical tests appropriate to the distribution of data (significance level, p <0.05). (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BERNACHI, Jéssica Santana. Monocyte-derived dendritic cell maturation and function from HIV-infected children and adolescents. 2014. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas Campinas, SP.