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Role of miR-1 in the mTORC1 signaling pathway in skeletal muscle

Grant number: 12/20183-0
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): January 07, 2013
Effective date (End): May 06, 2013
Field of knowledge:Health Sciences - Physical Education
Principal researcher:Maeli Dal Pai
Grantee:Ivan José Vechetti Júnior
Supervisor abroad: John J. McCarthy
Home Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
Research place: University of Kentucky (UK), United States  
Associated to the scholarship:11/14484-4 - MORPHOLOGICAL AND MOLECULAR RESPONSES IN SKELETAL MUSCLE OF AGED RATS SUBMITTED TO PHYSICAL TRAINING AFTER ATROPHYC STIMULUS, BP.DR

Abstract

Recent research advances in understanding the mechanisms and signaling pathways involved with the recovery process of muscle mass in senility, using different stimuli. These studies can contribute to the development of strategies to reduce or block the loss of muscle mass associated with aging. In this context, several studies have tried to investigate the signaling pathways and the role of Micro-RNAs involved in growth control, with maintenance of muscle phenotype as well as in understanding the process of recovery of muscle mass. This project aims to evaluate the role of microRNA (miR-1) in the regulation of hypertrophic signaling pathway (mTORC1) in skeletal muscle. Initially Bioinformatics tools will be used to identify possible targets of miR-1 in the mTORC1 pathway. After that, the genes will be validated targets of miR-1 by reporter gene construct 3'-UTR, with clone this region of the gene for luciferase. Each reporter gene is then co-transfected with miR-1 in fibroblast cells (3T3/NIH) and the activity of these genes is measured 48 hours later. For genes that show loss of expression is indicative that the 3'-UTR region contains a potential binding site miR-1. After this step, it is determined mRNA and protein expression of each target gene to determine if their pattern of expression is consistent with regulation of miRNA. This methodology will be held in Skeletal Muscle Biology Lab in the University of Kentucky, under the supervision of Prof.. Dr. John McCarthy. (AU)

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